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1i49

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Revision as of 08:43, 21 April 2021

CRYSTAL STRUCTURE ANALYSIS OF ARFAPTIN

Structural highlights

1i49 is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ARFP2_HUMAN] Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Small G proteins are GTP-dependent molecular switches that regulate numerous cellular functions. They can be classified into homologous subfamilies that are broadly associated with specific biological processes. Cross-talk between small G-protein families has an important role in signalling, but the mechanism by which it occurs is poorly understood. The coordinated action of Arf and Rho family GTPases is required to regulate many cellular processes including lipid signalling, cell motility and Golgi function. Arfaptin is a ubiquitously expressed protein implicated in mediating cross-talk between Rac (a member of the Rho family) and Arf small GTPases. Here we show that Arfaptin binds specifically to GTP-bound Arf1 and Arf6, but binds to Rac.GTP and Rac.GDP with similar affinities. The X-ray structure of Arfaptin reveals an elongated, crescent-shaped dimer of three-helix coiled-coils. Structures of Arfaptin with Rac bound to either GDP or the slowly hydrolysable analogue GMPPNP show that the switch regions adopt similar conformations in both complexes. Our data highlight fundamental differences between the molecular mechanisms of Rho and Ras family signalling, and suggest a model of Arfaptin-mediated synergy between the Arf and Rho family signalling pathways.

The structural basis of Arfaptin-mediated cross-talk between Rac and Arf signalling pathways.,Tarricone C, Xiao B, Justin N, Walker PA, Rittinger K, Gamblin SJ, Smerdon SJ Nature. 2001 May 10;411(6834):215-9. PMID:11346801^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tarricone C, Xiao B, Justin N, Walker PA, Rittinger K, Gamblin SJ, Smerdon SJ. The structural basis of Arfaptin-mediated cross-talk between Rac and Arf signalling pathways. Nature. 2001 May 10;411(6834):215-9. PMID:11346801 doi:http://dx.doi.org/10.1038/35075620

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1i49"

@@ Line 3: / Line 3: @@
 <StructureSection load='1i49' size='340' side='right'caption='[[1i49]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1i49]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I49 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I49 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1i49]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I49 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I49 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i49 OCA], [http://pdbe.org/1i49 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i49 RCSB], [http://www.ebi.ac.uk/pdbsum/1i49 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1i49 ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i49 OCA], [https://pdbe.org/1i49 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i49 RCSB], [https://www.ebi.ac.uk/pdbsum/1i49 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i49 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ARFP2_HUMAN ARFP2_HUMAN]] Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.
+[[https://www.uniprot.org/uniprot/ARFP2_HUMAN ARFP2_HUMAN]] Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

1i49

From Proteopedia

Revision as of 08:43, 21 April 2021

CRYSTAL STRUCTURE ANALYSIS OF ARFAPTIN

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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