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2fwr

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Revision as of 09:56, 5 May 2021

Structure of Archaeoglobus Fulgidis XPB

Structural highlights

2fwr is a 4 chain structure with sequence from Arcfl. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The human xeroderma pigmentosum group B (XPB) helicase is essential for transcription, nucleotide excision repair, and TFIIH functional assembly. Here, we determined crystal structures of an Archaeoglobus fulgidus XPB homolog (AfXPB) that characterize two RecA-like XPB helicase domains and discover a DNA damage recognition domain (DRD), a unique RED motif, a flexible thumb motif (ThM), and implied conformational changes within a conserved functional core. RED motif mutations dramatically reduce helicase activity, and the DRD and ThM, which flank the RED motif, appear structurally as well as functionally analogous to the MutS mismatch recognition and DNA polymerase thumb domains. Substrate specificity is altered by DNA damage, such that AfXPB unwinds dsDNA with 3' extensions, but not blunt-ended dsDNA, unless it contains a lesion, as shown for CPD or (6-4) photoproducts. Together, these results provide an unexpected mechanism of DNA unwinding with implications for XPB damage verification in nucleotide excision repair.

Conserved XPB core structure and motifs for DNA unwinding: implications for pathway selection of transcription or excision repair.,Fan L, Arvai AS, Cooper PK, Iwai S, Hanaoka F, Tainer JA Mol Cell. 2006 Apr 7;22(1):27-37. PMID:16600867^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fan L, Arvai AS, Cooper PK, Iwai S, Hanaoka F, Tainer JA. Conserved XPB core structure and motifs for DNA unwinding: implications for pathway selection of transcription or excision repair. Mol Cell. 2006 Apr 7;22(1):27-37. PMID:16600867 doi:http://dx.doi.org/10.1016/j.molcel.2006.02.017

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2fwr"

@@ Line 3: / Line 3: @@
 <StructureSection load='2fwr' size='340' side='right'caption='[[2fwr]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2fwr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfl Arcfl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FWR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FWR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2fwr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arcfl Arcfl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FWR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FWR FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fwr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fwr OCA], [http://pdbe.org/2fwr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fwr RCSB], [http://www.ebi.ac.uk/pdbsum/2fwr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fwr ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fwr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fwr OCA], [https://pdbe.org/2fwr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fwr RCSB], [https://www.ebi.ac.uk/pdbsum/2fwr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fwr ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==

2fwr

From Proteopedia

Revision as of 09:56, 5 May 2021

Structure of Archaeoglobus Fulgidis XPB

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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