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6o6y

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Current revision (14:50, 13 March 2024) (edit) (undo)
 
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<StructureSection load='6o6y' size='340' side='right'caption='[[6o6y]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
<StructureSection load='6o6y' size='340' side='right'caption='[[6o6y]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6o6y]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O6Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O6Y FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6o6y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_onnurineus Thermococcus onnurineus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O6Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6O6Y FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACK:(3AR,4R,6R,6AR)-4-(6-AMINO-9H-PURIN-9-YL)-6-(HYDROXYMETHYL)TETRAHYDROFURO[3,4-D][1,3,2]DIOXAPHOSPHOL-2-OL+2-OXIDE'>ACK</scene>, <scene name='pdbligand=LQJ:3-O-[(R)-{[(2S,3aS,4S,6S,6aS)-6-(6-amino-9H-purin-9-yl)-2-hydroxy-2-oxotetrahydro-2H-2lambda~5~-furo[3,4-d][1,3,2]dioxaphosphol-4-yl]methoxy}(hydroxy)phosphoryl]adenosine'>LQJ</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o6y OCA], [http://pdbe.org/6o6y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o6y RCSB], [http://www.ebi.ac.uk/pdbsum/6o6y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o6y ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACK:(3AR,4R,6R,6AR)-4-(6-AMINO-9H-PURIN-9-YL)-6-(HYDROXYMETHYL)TETRAHYDROFURO[3,4-D][1,3,2]DIOXAPHOSPHOL-2-OL+2-OXIDE'>ACK</scene>, <scene name='pdbligand=LQJ:3-O-[(R)-{[(2S,3aS,4S,6S,6aS)-6-(6-amino-9H-purin-9-yl)-2-hydroxy-2-oxotetrahydro-2H-2lambda~5~-furo[3,4-d][1,3,2]dioxaphosphol-4-yl]methoxy}(hydroxy)phosphoryl]adenosine'>LQJ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6o6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o6y OCA], [https://pdbe.org/6o6y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6o6y RCSB], [https://www.ebi.ac.uk/pdbsum/6o6y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6o6y ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/B6YWC3_THEON B6YWC3_THEON]
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Type III-A CRISPR-Cas surveillance complexes containing multi-subunit Csm effector, guide, and target RNAs exhibit multiple activities, including formation of cyclic-oligoadenylates (cAn) from ATP and subsequent cAn-mediated cleavage of single-strand RNA (ssRNA) by the trans-acting Csm6 RNase. Our structure-function studies have focused on Thermococcus onnurineus Csm6 to deduce mechanistic insights into how cA4 binding to the Csm6 CARF domain triggers the RNase activity of the Csm6 HEPN domain and what factors contribute to regulation of RNA cleavage activity. We demonstrate that the Csm6 CARF domain is a ring nuclease, whereby bound cA4 is stepwise cleaved initially to ApApApA&gt;p and subsequently to ApA&gt;p in its CARF domain-binding pocket, with such cleavage bursts using a timer mechanism to regulate the RNase activity of the Csm6 HEPN domain. In addition, we establish T. onnurineus Csm6 as an adenosine-specific RNase and identify a histidine in the cA4 CARF-binding pocket involved in autoinhibitory regulation of RNase activity.
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CRISPR-Cas III-A Csm6 CARF Domain Is a Ring Nuclease Triggering Stepwise cA4 Cleavage with ApA&gt;p Formation Terminating RNase Activity.,Jia N, Jones R, Yang G, Ouerfelli O, Patel DJ Mol Cell. 2019 Jun 28. pii: S1097-2765(19)30447-2. doi:, 10.1016/j.molcel.2019.06.014. PMID:31326273<ref>PMID:31326273</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6o6y" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Jia, N]]
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[[Category: Thermococcus onnurineus]]
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[[Category: Patel, D J]]
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[[Category: Jia N]]
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[[Category: Csm6 in complex with ca4 by cocrystallization of csm6 and ca4]]
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[[Category: Patel DJ]]
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[[Category: Immune system]]
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[[Category: Type iii-a crispr-cas system]]
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Current revision

Crystal structure of Csm6 in complex with cyclic-tetraadenylates (cA4) by cocrystallization of Csm6 and cA4

PDB ID 6o6y

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