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6nm5

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Revision as of 22:52, 6 March 2020

F-pilus/MS2 Maturation protein complex

6nm5, resolution 6.20Å

Structural highlights

6nm5 is a 76 chain structure with sequence from Enterobacteria phage ms2 and Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MAT_BPMS2] The maturation protein is required for the typical attachment of the phage to the side of the bacterial pili (PubMed:23810697). MP binds to sequences located toward each end of the genome, hence circularizing it (PubMed:26608810). The RNA genome-MP complex is released from the capsid upon host receptor binding (PubMed:23810697). MP enters the cell along with the viral RNA (PubMed:4551992).^[1] ^[2] ^[3]

Publication Abstract from PubMed

Single-stranded RNA bacteriophages (ssRNA phages) infect Gram-negative bacteria via a single maturation protein (Mat), which attaches to a retractile pilus of the host. Here we present structures of the ssRNA phage MS2 in complex with the Escherichia coli F-pilus, showing a network of hydrophobic and electrostatic interactions at the Mat-pilus interface. Moreover, binding of the pilus induces slight orientational variations of the Mat relative to the rest of the phage capsid, priming the Mat-connected genomic RNA (gRNA) for its release from the virions. The exposed tip of the attached Mat points opposite to the direction of the pilus retraction, which may facilitate the translocation of the gRNA from the capsid into the host cytosol. In addition, our structures determine the orientation of the assembled F-pilin subunits relative to the cell envelope, providing insights into the F-like type IV secretion systems.

Structural basis for the adsorption of a single-stranded RNA bacteriophage.,Meng R, Jiang M, Cui Z, Chang JY, Yang K, Jakana J, Yu X, Wang Z, Hu B, Zhang J Nat Commun. 2019 Jul 16;10(1):3130. doi: 10.1038/s41467-019-11126-8. PMID:31311931^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dent KC, Thompson R, Barker AM, Hiscox JA, Barr JN, Stockley PG, Ranson NA. The Asymmetric Structure of an Icosahedral Virus Bound to Its Receptor Suggests a Mechanism for Genome Release. Structure. 2013 Jun 25. pii: S0969-2126(13)00194-9. doi:, 10.1016/j.str.2013.05.012. PMID:23810697 doi:10.1016/j.str.2013.05.012
↑ Rolfsson O, Middleton S, Manfield IW, White SJ, Fan B, Vaughan R, Ranson NA, Dykeman E, Twarock R, Ford J, Kao CC, Stockley PG. Direct Evidence for Packaging Signal-Mediated Assembly of Bacteriophage MS2. J Mol Biol. 2016 Jan 29;428(2 Pt B):431-48. doi: 10.1016/j.jmb.2015.11.014. Epub , 2015 Dec 1. PMID:26608810 doi:http://dx.doi.org/10.1016/j.jmb.2015.11.014
↑ Krahn PM, O'Callaghan RJ, Paranchych W. Stages in phage R17 infection. VI. Injection of A protein and RNA into the host cell. Virology. 1972 Mar;47(3):628-37. PMID:4551992
↑ Meng R, Jiang M, Cui Z, Chang JY, Yang K, Jakana J, Yu X, Wang Z, Hu B, Zhang J. Structural basis for the adsorption of a single-stranded RNA bacteriophage. Nat Commun. 2019 Jul 16;10(1):3130. doi: 10.1038/s41467-019-11126-8. PMID:31311931 doi:http://dx.doi.org/10.1038/s41467-019-11126-8

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6nm5"

@@ Line 1: / Line 1: @@
 ==F-pilus/MS2 Maturation protein complex==
-<StructureSection load='6nm5' size='340' side='right'caption='[[6nm5]], [[Resolution|resolution]] 6.20&Aring;' scene=''>
+<SX load='6nm5' size='340' side='right' viewer='molstar' caption='[[6nm5]], [[Resolution|resolution]] 6.20&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[6nm5]] is a 76 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_ms2 Enterobacteria phage ms2] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NM5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NM5 FirstGlance]. <br>
@@ Line 21: / Line 21: @@
 <references/>
 __TOC__
-</StructureSection>
+</SX>
 [[Category: Enterobacteria phage ms2]]
 [[Category: Escherichia coli]]

6nm5

From Proteopedia

Revision as of 22:52, 6 March 2020

F-pilus/MS2 Maturation protein complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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