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2qop

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Revision as of 08:27, 25 June 2021

Crystal structure of the transcriptional regulator AcrR from Escherichia coli

Structural highlights

2qop is a 1 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ACRR_ECOLI] Potential regulator protein for the acrAB genes.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The AcrAB multidrug efflux pump, which belongs to the resistance nodulation division (RND) family, recognizes and extrudes a wide range of antibiotics and chemotherapeutic agents and causes the intrinsic antibiotic resistance in Escherichia coli. The expression of AcrAB is controlled by the transcriptional regulator AcrR, whose open reading frame is located 141 bp upstream of the acrAB operon. To understand the structural basis of AcrR regulation, we have determined the crystal structure of AcrR to 2.55-A resolution, revealing a dimeric two-domain molecule with an entirely helical architecture similar to members of the TetR family of transcriptional regulators. Each monomer of AcrR forms a multientrance pocket of 350 A(3) in the ligand-binding domain. The ligand-binding pocket is surrounded with mostly hydrophobic residues. In addition, a completely buried negatively charged glutamate, expected to be critical for drug binding, is located at the center of the binding pocket. The crystal structure provides novel insight into the mechanisms of ligand binding and AcrR regulation.

Crystal structure of the transcriptional regulator AcrR from Escherichia coli.,Li M, Gu R, Su CC, Routh MD, Harris KC, Jewell ES, McDermott G, Yu EW J Mol Biol. 2007 Nov 30;374(3):591-603. Epub 2007 Sep 29. PMID:17950313^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Li M, Gu R, Su CC, Routh MD, Harris KC, Jewell ES, McDermott G, Yu EW. Crystal structure of the transcriptional regulator AcrR from Escherichia coli. J Mol Biol. 2007 Nov 30;374(3):591-603. Epub 2007 Sep 29. PMID:17950313 doi:10.1016/j.jmb.2007.09.064

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2qop"

@@ Line 3: / Line 3: @@
 <StructureSection load='2qop' size='340' side='right'caption='[[2qop]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2qop]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QOP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2qop]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QOP FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qop OCA], [http://pdbe.org/2qop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qop RCSB], [http://www.ebi.ac.uk/pdbsum/2qop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2qop ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qop OCA], [https://pdbe.org/2qop PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qop RCSB], [https://www.ebi.ac.uk/pdbsum/2qop PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qop ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ACRR_ECOLI ACRR_ECOLI]] Potential regulator protein for the acrAB genes.
+[[https://www.uniprot.org/uniprot/ACRR_ECOLI ACRR_ECOLI]] Potential regulator protein for the acrAB genes.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 27: / Line 27: @@
 </div>
 <div class="pdbe-citations 2qop" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]]
+*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
 == References ==
 <references/>

2qop

From Proteopedia

Revision as of 08:27, 25 June 2021

Crystal structure of the transcriptional regulator AcrR from Escherichia coli

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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