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| | <StructureSection load='6hlk' size='340' side='right'caption='[[6hlk]], [[Resolution|resolution]] 2.42Å' scene=''> | | <StructureSection load='6hlk' size='340' side='right'caption='[[6hlk]], [[Resolution|resolution]] 2.42Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6hlk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HLK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HLK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6hlk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HLK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HLK FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.42Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CXXR01000010.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hlk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hlk OCA], [http://pdbe.org/6hlk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hlk RCSB], [http://www.ebi.ac.uk/pdbsum/6hlk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hlk ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hlk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hlk OCA], [https://pdbe.org/6hlk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hlk RCSB], [https://www.ebi.ac.uk/pdbsum/6hlk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hlk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A5H1ZR32_ECOLX A0A5H1ZR32_ECOLX] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Alqasmi, M]] | + | [[Category: Alqasmi M]] |
| - | [[Category: Bacarizo, J]] | + | [[Category: Bacarizo J]] |
| - | [[Category: Ciges-Tomas, J R]] | + | [[Category: Ciges-Tomas JR]] |
| - | [[Category: Fillol-Salom, A]] | + | [[Category: Fillol-Salom A]] |
| - | [[Category: Marina, A]] | + | [[Category: Marina A]] |
| - | [[Category: Penades, J R]] | + | [[Category: Penades JR]] |
| - | [[Category: Roszak, A W]] | + | [[Category: Roszak AW]] |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Homo-dimer]]
| + | |
| - | [[Category: Phage interference]]
| + | |
| - | [[Category: Redirecting packaging protein]]
| + | |
| Structural highlights
Function
A0A5H1ZR32_ECOLX
Publication Abstract from PubMed
Phage-inducible chromosomal islands (PICIs) represent a novel and universal class of mobile genetic elements, which have broad impact on bacterial virulence. In spite of their relevance, how the Gram-negative PICIs hijack the phage machinery for their own specific packaging and how they block phage reproduction remains to be determined. Using genetic and structural analyses, we solve the mystery here by showing that the Gram-negative PICIs encode a protein that simultaneously performs these processes. This protein, which we have named Rpp (for redirecting phage packaging), interacts with the phage terminase small subunit, forming a heterocomplex. This complex is unable to recognize the phage DNA, blocking phage packaging, but specifically binds to the PICI genome, promoting PICI packaging. Our studies reveal the mechanism of action that allows PICI dissemination in nature, introducing a new paradigm in the understanding of the biology of pathogenicity islands and therefore of bacterial pathogen evolution.
Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit.,Fillol-Salom A, Bacarizo J, Alqasmi M, Ciges-Tomas JR, Martinez-Rubio R, Roszak AW, Cogdell RJ, Chen J, Marina A, Penades JR Mol Cell. 2019 Jul 8. pii: S1097-2765(19)30473-3. doi:, 10.1016/j.molcel.2019.06.017. PMID:31350119[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fillol-Salom A, Bacarizo J, Alqasmi M, Ciges-Tomas JR, Martinez-Rubio R, Roszak AW, Cogdell RJ, Chen J, Marina A, Penades JR. Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit. Mol Cell. 2019 Jul 8. pii: S1097-2765(19)30473-3. doi:, 10.1016/j.molcel.2019.06.017. PMID:31350119 doi:http://dx.doi.org/10.1016/j.molcel.2019.06.017
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