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| | ==Human Guanylate Kinase== | | ==Human Guanylate Kinase== |
| - | <StructureSection load='6nui' size='340' side='right'caption='[[6nui]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='6nui' size='340' side='right'caption='[[6nui]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6nui]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NUI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NUI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6nui]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NUI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NUI FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GUK1, GMK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Guanylate_kinase Guanylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.8 2.7.4.8] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nui OCA], [https://pdbe.org/6nui PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nui RCSB], [https://www.ebi.ac.uk/pdbsum/6nui PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nui ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nui OCA], [http://pdbe.org/6nui PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nui RCSB], [http://www.ebi.ac.uk/pdbsum/6nui PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nui ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/KGUA_HUMAN KGUA_HUMAN]] Essential for recycling GMP and indirectly, cGMP. | + | [https://www.uniprot.org/uniprot/KGUA_HUMAN KGUA_HUMAN] Essential for recycling GMP and indirectly, cGMP. |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Human guanylate kinase (hGMPK) is the only known enzyme responsible for cellular GDP production, making it essential for cellular viability and proliferation. Moreover, hGMPK has been assigned a critical role in metabolic activation of antiviral and antineoplastic nucleoside-analog pro-drugs. Given that hGMPK is indispensable for producing the nucleotide building blocks of DNA, RNA, and cGMP and that cancer cells possess elevated GTP levels, it is surprising that a detailed structural and functional characterization of hGMPK is lacking. Here, we present the first high-resolution structure of hGMPK in the apo form, determined with NMR spectroscopy. The structure revealed that hGMPK consists of three distinct regions designated as the LID, GMP-binding (GMP-BD), and CORE domains and is in an open configuration that is nucleotide-binding competent. We also demonstrate that nonsynonymous single-nucleotide variants (nsSNVs) of the hGMPK CORE domain distant from the nucleotide-binding site of this domain modulate enzymatic activity without significantly affecting hGMPK's structure. Finally, we show that knocking down the hGMPK gene in lung adenocarcinoma cell lines decreases cellular viability, proliferation, and clonogenic potential while not altering the proliferation of immortalized, non-cancerous human peripheral airway cells. Taken together, our results provide an important step toward establishing hGMPK as a potential biomolecular target, from both an orthosteric (ligand-binding sites) and allosteric (location of CORE domain-located nsSNVs) standpoint.
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| - | Solution structure and functional investigation of human guanylate kinase reveals allosteric networking and a crucial role for the enzyme in cancer.,Khan N, Shah PP, Ban D, Trigo-Mourino P, Carneiro MG, DeLeeuw L, Dean WL, Trent JO, Beverly LJ, Konrad M, Lee D, Sabo TM J Biol Chem. 2019 Jun 14. pii: RA119.009251. doi: 10.1074/jbc.RA119.009251. PMID:31201273<ref>PMID:31201273</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 6nui" style="background-color:#fffaf0;"></div>
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| | ==See Also== | | ==See Also== |
| | *[[Guanylate kinase 3D structures|Guanylate kinase 3D structures]] | | *[[Guanylate kinase 3D structures|Guanylate kinase 3D structures]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Guanylate kinase]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Human]]
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ban, D]] | + | [[Category: Ban D]] |
| - | [[Category: Carneiro, M G]] | + | [[Category: Carneiro MG]] |
| - | [[Category: Khan, N]] | + | [[Category: Khan N]] |
| - | [[Category: Konrad, M]] | + | [[Category: Konrad M]] |
| - | [[Category: Lee, D]] | + | [[Category: Lee D]] |
| - | [[Category: Sabo, T M]] | + | [[Category: Sabo TM]] |
| - | [[Category: Trent, J O]] | + | [[Category: Trent JO]] |
| - | [[Category: Trigo-Mourino, P]] | + | [[Category: Trigo-Mourino P]] |
| - | [[Category: Atp:gmp-phosphotransferase]]
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| - | [[Category: Gmp kinase]]
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| - | [[Category: Purine metabolism]]
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| - | [[Category: Transferase]]
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| - | [[Category: Unliganded]]
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