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| | <StructureSection load='6mrk' size='340' side='right'caption='[[6mrk]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='6mrk' size='340' side='right'caption='[[6mrk]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6mrk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MRK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MRK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6mrk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MRK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MRK FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nxf2, CG4118 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME]), Nxt1, CG12752 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mrk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mrk OCA], [http://pdbe.org/6mrk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mrk RCSB], [http://www.ebi.ac.uk/pdbsum/6mrk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mrk ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mrk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mrk OCA], [https://pdbe.org/6mrk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mrk RCSB], [https://www.ebi.ac.uk/pdbsum/6mrk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mrk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NXF2_DROME NXF2_DROME]] May be involved in the export of mRNA from the nucleus to the cytoplasm.<ref>PMID:11780633</ref> [[http://www.uniprot.org/uniprot/NXT1_DROME NXT1_DROME]] Stimulator of protein export for NES-containing proteins (By similarity). Plays a role in the nuclear export of mRNA (PubMed:14729961). Also plays a role in the nuclear export of U1 snRNA, tRNA, and mRNA (By similarity).[UniProtKB:Q9UKK6]<ref>PMID:14729961</ref> | + | [https://www.uniprot.org/uniprot/NXF2_DROME NXF2_DROME] May be involved in the export of mRNA from the nucleus to the cytoplasm.<ref>PMID:11780633</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Patel, D J]] | + | [[Category: Patel DJ]] |
| - | [[Category: Wang, J]] | + | [[Category: Wang J]] |
| - | [[Category: Heterochromatin formation]]
| + | |
| - | [[Category: Pirna pathway]]
| + | |
| - | [[Category: Piwi]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Transcriptional silencing]]
| + | |
| - | [[Category: Transposon silencing]]
| + | |
| Structural highlights
Function
NXF2_DROME May be involved in the export of mRNA from the nucleus to the cytoplasm.[1]
Publication Abstract from PubMed
The PIWI-interacting RNA (piRNA) pathway protects genome integrity in part through establishing repressive heterochromatin at transposon loci. Silencing requires piRNA-guided targeting of nuclear PIWI proteins to nascent transposon transcripts, yet the subsequent molecular events are not understood. Here, we identify SFiNX (silencing factor interacting nuclear export variant), an interdependent protein complex required for Piwi-mediated cotranscriptional silencing in Drosophila. SFiNX consists of Nxf2-Nxt1, a gonad-specific variant of the heterodimeric messenger RNA export receptor Nxf1-Nxt1 and the Piwi-associated protein Panoramix. SFiNX mutant flies are sterile and exhibit transposon derepression because piRNA-loaded Piwi is unable to establish heterochromatin. Within SFiNX, Panoramix recruits heterochromatin effectors, while the RNA binding protein Nxf2 licenses cotranscriptional silencing. Our data reveal how Nxf2 might have evolved from an RNA transport receptor into a cotranscriptional silencing factor. Thus, NXF variants, which are abundant in metazoans, can have diverse molecular functions and might have been coopted for host genome defense more broadly.
The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation.,Batki J, Schnabl J, Wang J, Handler D, Andreev VI, Stieger CE, Novatchkova M, Lampersberger L, Kauneckaite K, Xie W, Mechtler K, Patel DJ, Brennecke J Nat Struct Mol Biol. 2019 Aug;26(8):720-731. doi: 10.1038/s41594-019-0270-6. Epub, 2019 Aug 5. PMID:31384064[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Herold A, Klymenko T, Izaurralde E. NXF1/p15 heterodimers are essential for mRNA nuclear export in Drosophila. RNA. 2001 Dec;7(12):1768-80. PMID:11780633
- ↑ Batki J, Schnabl J, Wang J, Handler D, Andreev VI, Stieger CE, Novatchkova M, Lampersberger L, Kauneckaite K, Xie W, Mechtler K, Patel DJ, Brennecke J. The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation. Nat Struct Mol Biol. 2019 Aug;26(8):720-731. doi: 10.1038/s41594-019-0270-6. Epub, 2019 Aug 5. PMID:31384064 doi:http://dx.doi.org/10.1038/s41594-019-0270-6
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