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| | <StructureSection load='6m7y' size='340' side='right'caption='[[6m7y]], [[Resolution|resolution]] 2.79Å' scene=''> | | <StructureSection load='6m7y' size='340' side='right'caption='[[6m7y]], [[Resolution|resolution]] 2.79Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6m7y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"lactococcus_lactis_subsp._diacitilactis_(sic)"_(garvie_and_farrow_1982)_schleifer_et_al._1986 "lactococcus lactis subsp. diacitilactis (sic)" (garvie and farrow 1982) schleifer et al. 1986]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M7Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6M7Y FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6m7y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] and [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis Lactococcus lactis subsp. lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M7Y FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=J9A:'>J9A</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.794Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nisB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1360 "Lactococcus lactis subsp. diacitilactis (sic)" (Garvie and Farrow 1982) Schleifer et al. 1986])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=J9A:N-[(2S)-2-amino-2-carboxyethyl]-L-alpha-glutamine'>J9A</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6m7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m7y OCA], [http://pdbe.org/6m7y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m7y RCSB], [http://www.ebi.ac.uk/pdbsum/6m7y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m7y ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m7y OCA], [https://pdbe.org/6m7y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m7y RCSB], [https://www.ebi.ac.uk/pdbsum/6m7y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m7y ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NISB_LACLL NISB_LACLL]] Involved in the post-translational modification of the lantibiotic nisin. [[http://www.uniprot.org/uniprot/Q7BB86_9LACT Q7BB86_9LACT]] Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.[RuleBase:RU362078] | + | [https://www.uniprot.org/uniprot/NISB_LACLL NISB_LACLL] Involved in the post-translational modification of the lantibiotic nisin. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Lactococcus lactis]] |
| | + | [[Category: Lactococcus lactis subsp. lactis]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cogan, D P]] | + | [[Category: Cogan DP]] |
| - | [[Category: Nair, S K]] | + | [[Category: Nair SK]] |
| - | [[Category: Biosynthetic protein]]
| + | |
| - | [[Category: Dehydratase]]
| + | |
| - | [[Category: Nisin]]
| + | |
| - | [[Category: Ripp]]
| + | |
| - | [[Category: Trna-dependent]]
| + | |
| Structural highlights
Function
NISB_LACLL Involved in the post-translational modification of the lantibiotic nisin.
Publication Abstract from PubMed
The peptide natural product nisin has been used as a food preservative for 6 decades with minimal development of resistance. Nisin contains the unusual amino acids dehydroalanine and dehydrobutyrine, which are posttranslationally installed by class I lanthipeptide dehydratases (LanBs) on a linear peptide substrate through an unusual glutamyl-tRNA-dependent dehydration of Ser and Thr. To date, little is known about how LanBs catalyze the transfer of glutamate from charged tRNA(Glu) to the peptide substrate, or how they carry out the subsequent elimination of the peptide-glutamyl adducts to afford dehydro amino acids. Here, we describe the synthesis of inert analogs that mimic substrate glutamyl-tRNA(Glu) and the glutamylated peptide intermediate, and determine the crystal structures of 2 LanBs in complex with each of these compounds. Mutational studies were used to characterize the function of the glutamylation and glutamate elimination active-site residues identified through the structural analysis. These combined studies provide insights into the mechanisms of substrate recognition, glutamylation, and glutamate elimination by LanBs to effect a net dehydration reaction of Ser and Thr.
Characterization of glutamyl-tRNA-dependent dehydratases using nonreactive substrate mimics.,Bothwell IR, Cogan DP, Kim T, Reinhardt CJ, van der Donk WA, Nair SK Proc Natl Acad Sci U S A. 2019 Aug 13. pii: 1905240116. doi:, 10.1073/pnas.1905240116. PMID:31409709[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bothwell IR, Cogan DP, Kim T, Reinhardt CJ, van der Donk WA, Nair SK. Characterization of glutamyl-tRNA-dependent dehydratases using nonreactive substrate mimics. Proc Natl Acad Sci U S A. 2019 Aug 13. pii: 1905240116. doi:, 10.1073/pnas.1905240116. PMID:31409709 doi:http://dx.doi.org/10.1073/pnas.1905240116
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