This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


6p29

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:53, 13 March 2024) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='6p29' size='340' side='right'caption='[[6p29]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
<StructureSection load='6p29' size='340' side='right'caption='[[6p29]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[6p29]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"alteromonas_luteo-violaceus"_(sic)_gauthier_1976 "alteromonas luteo-violaceus" (sic) gauthier 1976]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P29 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P29 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[6p29]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudoalteromonas_luteoviolacea Pseudoalteromonas luteoviolacea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P29 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6P29 FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NQ7:(5S)-2-amino-5-[(1R)-1-(1H-indol-3-yl)ethyl]-1,3-oxazol-4(5H)-one'>NQ7</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">N473_05385 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43657 "Alteromonas luteo-violaceus" (sic) Gauthier 1976])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NQ7:(5S)-2-amino-5-[(1R)-1-(1H-indol-3-yl)ethyl]-1,3-oxazol-4(5H)-one'>NQ7</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6p29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p29 OCA], [http://pdbe.org/6p29 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p29 RCSB], [http://www.ebi.ac.uk/pdbsum/6p29 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p29 ProSAT]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6p29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p29 OCA], [https://pdbe.org/6p29 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6p29 RCSB], [https://www.ebi.ac.uk/pdbsum/6p29 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6p29 ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/A0A167HII1_9GAMM A0A167HII1_9GAMM]
-
Microbes produce specialized metabolites to thrive in their natural habitats. However, it is rare that a given specialized metabolite is biosynthesized via pathways with distinct intermediates and enzymes. Here, we show that the core assembly mechanism of the antibiotic indolmycin in marine gram-negative Pseudoalteromonas luteoviolacea is distinct from its counterpart in terrestrial gram-positive Streptomyces species, with a molecule that is a shunt product in the Streptomyces pathway employed as a biosynthetic substrate for a novel metal-independent N-demethylindolmycin synthase in the P. luteoviolacea pathway. To provide insight into this reaction, we solved the 1.5 A resolution structure in complex with product and identified the active site residues. Guided by our biosynthetic insights, we then engineered the Streptomyces indolmycin producer for titer improvement. This study provides a paradigm for understanding how two unique routes to a microbial specialized metabolite can emerge from convergent biosynthetic transformations.
+
-
 
+
-
Convergent biosynthetic transformations to a bacterial specialized metabolite.,Du YL, Higgins MA, Zhao G, Ryan KS Nat Chem Biol. 2019 Aug 12. pii: 10.1038/s41589-019-0331-5. doi:, 10.1038/s41589-019-0331-5. PMID:31406372<ref>PMID:31406372</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 6p29" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Du, Y L]]
+
[[Category: Pseudoalteromonas luteoviolacea]]
-
[[Category: Higgins, M A]]
+
[[Category: Du YL]]
-
[[Category: Ryan, K S]]
+
[[Category: Higgins MA]]
-
[[Category: Zhao, G]]
+
[[Category: Ryan KS]]
-
[[Category: Biosynthetic protein]]
+
[[Category: Zhao G]]
-
[[Category: N-demethylindolmycin synthase]]
+

Current revision

N-demethylindolmycin synthase (PluN2) in complex with N-demethylindolmycin

PDB ID 6p29

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6p29"
Personal tools