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| | <StructureSection load='4dvo' size='340' side='right'caption='[[4dvo]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='4dvo' size='340' side='right'caption='[[4dvo]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4dvo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DVO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DVO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4dvo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DVO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DVO FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SOR:D-SORBITOL'>SOR</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SOR:D-SORBITOL'>SOR</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4duo|4duo]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dvo OCA], [https://pdbe.org/4dvo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dvo RCSB], [https://www.ebi.ac.uk/pdbsum/4dvo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dvo ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Xylose_isomerase Xylose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.5 5.3.1.5] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dvo OCA], [http://pdbe.org/4dvo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dvo RCSB], [http://www.ebi.ac.uk/pdbsum/4dvo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dvo ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/XYLA_STRRU XYLA_STRRU]] Involved in D-xylose catabolism. | + | [[https://www.uniprot.org/uniprot/XYLA_STRRU XYLA_STRRU]] Involved in D-xylose catabolism. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Streptomyces rubiginosus]] | | [[Category: Streptomyces rubiginosus]] |
| - | [[Category: Xylose isomerase]]
| + | [[Category: Hanson L]] |
| - | [[Category: Hanson, L]] | + | [[Category: Kovalevsky AY]] |
| - | [[Category: Kovalevsky, A Y]] | + | [[Category: Langan P]] |
| - | [[Category: Langan, P]] | + | |
| - | [[Category: Isomerase]]
| + | |
| - | [[Category: Tim-barrel]]
| + | |
| Structural highlights
Function
[XYLA_STRRU] Involved in D-xylose catabolism.
Publication Abstract from PubMed
D-Xylose isomerase (XI) converts the aldo-sugars xylose and glucose to their keto analogs xylulose and fructose, but is strongly inhibited by the polyols xylitol and sorbitol, especially at acidic pH. In order to understand the atomic details of polyol binding to the XI active site, a 2.0 A resolution room-temperature joint X-ray/neutron structure of XI in complex with Ni(2+) cofactors and sorbitol inhibitor at pH 5.9 and a room-temperature X-ray structure of XI containing Mg(2+) ions and xylitol at the physiological pH of 7.7 were obtained. The protonation of oxygen O5 of the inhibitor, which was found to be deprotonated and negatively charged in previous structures of XI complexed with linear glucose and xylulose, was directly observed. The Ni(2+) ions occupying the catalytic metal site (M2) were found at two locations, while Mg(2+) in M2 is very mobile and has a high B factor. Under acidic conditions sorbitol gains a water-mediated interaction that connects its O1 hydroxyl to Asp257. This contact is not found in structures at basic pH. The new interaction that is formed may improve the binding of the inhibitor, providing an explanation for the increased affinity of the polyols for XI at low pH.
Inhibition of D-xylose isomerase by polyols: atomic details by joint X-ray/neutron crystallography.,Kovalevsky A, Hanson BL, Mason SA, Forsyth VT, Fisher Z, Mustyakimov M, Blakeley MP, Keen DA, Langan P Acta Crystallogr D Biol Crystallogr. 2012 Sep;68(Pt 9):1201-6. Epub 2012 Aug 18. PMID:22948921[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kovalevsky A, Hanson BL, Mason SA, Forsyth VT, Fisher Z, Mustyakimov M, Blakeley MP, Keen DA, Langan P. Inhibition of D-xylose isomerase by polyols: atomic details by joint X-ray/neutron crystallography. Acta Crystallogr D Biol Crystallogr. 2012 Sep;68(Pt 9):1201-6. Epub 2012 Aug 18. PMID:22948921 doi:http://dx.doi.org/10.1107/S0907444912024808
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