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6r2n

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Revision as of 07:27, 30 September 2020

Crystal structure of KlGlk1 glucokinase from Kluyveromyces lactis

Structural highlights

6r2n is a 3 chain structure with sequence from Atcc 56498. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	GLK1, KLLA0C01155g (ATCC 56498)
Activity:	Glucokinase, with EC number 2.7.1.2
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HXKG_KLULA] Glukokinase specific for aldohexoses. Phosphorylates glucose and mannose, but not fructose.^[1]

Publication Abstract from PubMed

Glucose phosphorylating enzymes are crucial in the regulation of basic cellular processes, including metabolism and gene expression. Glucokinases and hexokinases provide a pool of phosphorylated glucose in an adenosine diphosphate (ADP)- and ATP-dependent manner to shape the cell metabolism. The glucose processing enzymes from Kluyveromyces lactis are poorly characterized despite the emerging contribution of this yeast strain to industrial and laboratory scale biotechnology. The first reports on K. lactis glucokinase (KlGlk1) positioned the enzyme as an essential component required for glucose signaling. Nevertheless, no biochemical and structural information was available until now. Here, we present the first crystal structure of KlGlk1 together with biochemical characterization, including substrate specificity and enzyme kinetics. Additionally, comparative analysis of the presented structure and the prior structures of lactis hexokinase (KlHxk1) demonstrates the potential transitions between open and closed enzyme conformations upon ligand binding.

Crystal Structure of Kluyveromyces lactis Glucokinase (KlGlk1).,Zak KM, Kalinska M, Wator E, Kuska K, Krutyholowa R, Dubin G, Popowicz GM, Grudnik P Int J Mol Sci. 2019 Sep 28;20(19). pii: ijms20194821. doi: 10.3390/ijms20194821. PMID:31569356^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kettner K, Muller EC, Otto A, Rodel G, Breunig KD, Kriegel TM. Identification and characterization of a novel glucose-phosphorylating enzyme in Kluyveromyces lactis. FEMS Yeast Res. 2007 Aug;7(5):683-92. doi: 10.1111/j.1567-1364.2007.00259.x. Epub, 2007 Jun 16. PMID:17573926 doi:http://dx.doi.org/10.1111/j.1567-1364.2007.00259.x
↑ Zak KM, Kalinska M, Wator E, Kuska K, Krutyholowa R, Dubin G, Popowicz GM, Grudnik P. Crystal Structure of Kluyveromyces lactis Glucokinase (KlGlk1). Int J Mol Sci. 2019 Sep 28;20(19). pii: ijms20194821. doi: 10.3390/ijms20194821. PMID:31569356 doi:http://dx.doi.org/10.3390/ijms20194821

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6r2n"

@@ Line 3: / Line 3: @@
 <StructureSection load='6r2n' size='340' side='right'caption='[[6r2n]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6r2n]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R2N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R2N FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6r2n]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R2N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6R2N FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GLK1, KLLA0C01155g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28985 ATCC 56498])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r2n OCA], [http://pdbe.org/6r2n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r2n RCSB], [http://www.ebi.ac.uk/pdbsum/6r2n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r2n ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6r2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r2n OCA], [http://pdbe.org/6r2n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r2n RCSB], [http://www.ebi.ac.uk/pdbsum/6r2n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r2n ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 24: @@
 __TOC__
 </StructureSection>
+[[Category: Atcc 56498]]
 [[Category: Glucokinase]]
 [[Category: Large Structures]]

6r2n

From Proteopedia

Revision as of 07:27, 30 September 2020

Crystal structure of KlGlk1 glucokinase from Kluyveromyces lactis

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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