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| | <StructureSection load='5mqc' size='340' side='right'caption='[[5mqc]], [[Resolution|resolution]] 3.40Å' scene=''> | | <StructureSection load='5mqc' size='340' side='right'caption='[[5mqc]], [[Resolution|resolution]] 3.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5mqc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Black_queen_cell_virus Black queen cell virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MQC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MQC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5mqc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Black_queen_cell_virus Black queen cell virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MQC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MQC FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mqc OCA], [http://pdbe.org/5mqc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mqc RCSB], [http://www.ebi.ac.uk/pdbsum/5mqc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mqc ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mqc OCA], [https://pdbe.org/5mqc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mqc RCSB], [https://www.ebi.ac.uk/pdbsum/5mqc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mqc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9J7C2_9VIRU Q9J7C2_9VIRU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Black queen cell virus]] | | [[Category: Black queen cell virus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Kiem, H H.T]] | + | [[Category: Kiem HHT]] |
| - | [[Category: Plevka, P]] | + | [[Category: Plevka P]] |
| - | [[Category: Spurny, R]] | + | [[Category: Spurny R]] |
| - | [[Category: Apis mellifera]]
| + | |
| - | [[Category: Capsid]]
| + | |
| - | [[Category: Cripavirus]]
| + | |
| - | [[Category: Dicistroviridae]]
| + | |
| - | [[Category: Honey bee]]
| + | |
| - | [[Category: Honeybee]]
| + | |
| - | [[Category: Picornavirale]]
| + | |
| - | [[Category: Virion]]
| + | |
| - | [[Category: Virus]]
| + | |
| Structural highlights
Function
Q9J7C2_9VIRU
Publication Abstract from PubMed
Viral diseases are a major threat to honeybee (Apis mellifera) populations world-wide and therefore an important factor in reliable crop pollination and food security. Black queen cell virus (BQCV) is the etiological agent of a fatal disease of honeybee queen larvae and pupae. The virus belongs to the genus Triatovirus from the family Dicistroviridae that is part of the order Picornavirales Here we present a crystal structure of BQCV determined to a resolution of 3.4 A. The virion is formed by sixty copies of each of the major capsid proteins VP1, VP2, and VP3, however there is no density corresponding to a 75-residue-long minor capsid protein VP4 encoded by the BQCV genome. We show that the VP4 subunits are present in the crystallized virions that are infectious. This aspect of the BQCV virion is similar to that of the previously characterized triatoma virus and supports the recent establishment of the separate genus Triatovirus within the family Dicistroviridae The C-terminus of VP1 and CD loops of capsid proteins VP1 and VP3 of BQCV form 34-A-tall finger-like protrusions at the virion surface. The protrusions are larger than those of related dicistroviruses. IMPORTANCE: The western honeybee is the most important pollinator of all, and is required to sustain the agricultural production and biodiversity of wild flowering plants. However, honeybee populations worldwide are suffering from virus infections that cause colony losses. One of the most common, and least known, honeybee pathogens is black queen cell virus (BQCV), which at high titers causes queen larvae and pupae to turn black and die. Here we present the three-dimensional virion structure of BQCV determined by X-ray crystallography. The structure of BQCV reveals large protrusions on the virion surface. Capsid protein VP1 of BQCV does not contain a hydrophobic pocket. Therefore, the BQCV virion structure provides evidence that capsid-binding antiviral compounds that can prevent the replication of vertebrate picornaviruses may be ineffective against honeybee virus infections.
Virion structure of black queen cell virus, a common honeybee pathogen.,Spurny R, Pridal A, Palkova L, Tran Kiem HK, de Miranda JR, Plevka P J Virol. 2017 Jan 11. pii: JVI.02100-16. doi: 10.1128/JVI.02100-16. PMID:28077635[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Spurny R, Pridal A, Palkova L, Tran Kiem HK, de Miranda JR, Plevka P. Virion structure of black queen cell virus, a common honeybee pathogen. J Virol. 2017 Jan 11. pii: JVI.02100-16. doi: 10.1128/JVI.02100-16. PMID:28077635 doi:http://dx.doi.org/10.1128/JVI.02100-16
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