Luciferase
From Proteopedia
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== Relevance == | == Relevance == | ||
| - | Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success<ref>PMID:3072883</ref>. | + | Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success<ref>PMID:3072883</ref>. '''NanoLuc luciferase''' is a small luciferase with enhanced stability and a significant increase in luminescence <ref>PMID:27045664</ref>. |
== 3D Structures of Luciferase == | == 3D Structures of Luciferase == | ||
Revision as of 09:47, 18 June 2023
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Contents |
Additional information
(2d1s)
Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.
- For additional information, see: Colored & Bioluminescent Proteins
References
- ↑ Meighen EA. Molecular biology of bacterial bioluminescence. Microbiol Rev. 1991 Mar;55(1):123-42. PMID:2030669
- ↑ Gould SJ, Subramani S. Firefly luciferase as a tool in molecular and cell biology. Anal Biochem. 1988 Nov 15;175(1):5-13. PMID:3072883
- ↑ England CG, Ehlerding EB, Cai W. NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. Bioconjug Chem. 2016 May 18;27(5):1175-1187. PMID:27045664 doi:10.1021/acs.bioconjchem.6b00112
Additional Literature
- Marques SM, Esteves da Silva JC. Firefly bioluminescence: a mechanistic approach of luciferase catalyzed reactions. IUBMB Life. 2009 Jan;61(1):6-17. PMID:18949818 doi:10.1002/iub.134
- Inouye S. Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions. Cell Mol Life Sci. 2010 Feb;67(3):387-404. Epub 2009 Oct 27. PMID:19859663 doi:10.1007/s00018-009-0170-8
External Resources
- Yahoo news coverage of luciferase studies funded research for military applications
- Luciferase at Wikipedia
- Luciferase: June 2006 Molecule of the Month as part of the series of tutorials that are at the RCSB Protein Data Bank and written by David S. Goodsell
Proteopedia Page Contributors and Editors (what is this?)
Michal Harel, Wayne Decatur, Alexander Berchansky, Loïc Gazquez, Charlene Planchenault, David Canner
