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| | <StructureSection load='6prg' size='340' side='right'caption='[[6prg]], [[Resolution|resolution]] 2.35Å' scene=''> | | <StructureSection load='6prg' size='340' side='right'caption='[[6prg]], [[Resolution|resolution]] 2.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6prg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PRG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PRG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6prg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PRG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PRG FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SP_1897 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=170187 STRPN])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6prg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6prg OCA], [http://pdbe.org/6prg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6prg RCSB], [http://www.ebi.ac.uk/pdbsum/6prg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6prg ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6prg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6prg OCA], [https://pdbe.org/6prg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6prg RCSB], [https://www.ebi.ac.uk/pdbsum/6prg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6prg ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0H2URJ7_STRPN A0A0H2URJ7_STRPN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Strpn]] | + | [[Category: Streptococcus pneumoniae TIGR4]] |
| - | [[Category: Boraston, A B]] | + | [[Category: Boraston AB]] |
| - | [[Category: Meier, E P.W]] | + | [[Category: Meier EPW]] |
| - | [[Category: Cluster d-i solute binding protein]]
| + | |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Sbp]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
6prg is a 2 chain structure with sequence from Streptococcus pneumoniae TIGR4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 2.35Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
A0A0H2URJ7_STRPN
Publication Abstract from PubMed
Streptococcus pneumoniae is an opportunistic respiratory pathogen that can spread to other body sites, including the ears, brain and blood. The ability of this bacterium to breakdown, import and metabolize a wide range of glycans is key to its virulence. Intriguingly, S. pneumoniae can utilize several plant oligosaccharides for growth in vitro, including raffinose-family oligosaccharides (RFOs, which are alpha-(1-->6)-galactosyl extensions of sucrose). An RFO utilization locus has been identified in the pneumococcal genome; however, none of the proteins encoded by this locus have been biochemically characterized. The enigmatic ability of S. pneumoniae to utilize RFOs has recently received attention as mutations in two of the RFO locus genes have been linked to the tissue tropism of clinical pneumococcal isolates. Here, we use functional studies combined with X-ray crystallography to show that, while the pneumococcal RFO locus encodes for all the machinery required for uptake and degradation of RFOs, the individual pathway components are biochemically inefficient. We also demonstrate that the initiating enzyme in this pathway, the alpha-galactosidase Aga (a family 36 glycoside hydrolase), can cleave alpha-(1-->3)-linked galactose units from a linear blood group antigen. We propose that the pneumococcal RFO pathway is an evolutionary relic that is not utilized in this streptococcal species and, as such, is under no selection pressure to maintain binding affinity and/or catalytic efficiency. We speculate that the apparent contribution of RFO utilization to pneumococcal tissue tropism may, in fact, be due to the essential role the ATPase RafK plays in the transport of other carbohydrates.
Molecular analysis of an enigmatic Streptococcus pneumoniae virulence factor: the raffinose-family oligosaccharide utilization system.,Hobbs JK, Meier EPW, Pluvinage B, Mey MA, Boraston AB J Biol Chem. 2019 Oct 7. pii: RA119.010280. doi: 10.1074/jbc.RA119.010280. PMID:31591266[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hobbs JK, Meier EPW, Pluvinage B, Mey MA, Boraston AB. Molecular analysis of an enigmatic Streptococcus pneumoniae virulence factor: the raffinose-family oligosaccharide utilization system. J Biol Chem. 2019 Oct 7. pii: RA119.010280. doi: 10.1074/jbc.RA119.010280. PMID:31591266 doi:http://dx.doi.org/10.1074/jbc.RA119.010280
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