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1eww

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Revision as of 11:08, 28 July 2021

SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEGREES CELSIUS

Structural highlights

1eww is a 1 chain structure with sequence from Archips fumiferana. The December 2009 RCSB PDB Molecule of the Month feature on Antifreeze Proteins by David Goodsell is 10.2210/rcsb_pdb/mom_2009_12. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Insect antifreeze proteins (AFP) are considerably more active at inhibiting ice crystal growth than AFP from fish or plants. Several insect AFPs, also known as thermal hysteresis proteins, have been cloned and expressed. Their maximum activity is 3-4 times that of fish AFPs and they are 10-100 times more effective at micromolar concentrations. Here we report the solution structure of spruce budworm (Choristoneura fumiferana) AFP and characterize its ice-binding properties. The 9-kDa AFP is a beta-helix with a triangular cross-section and rectangular sides that form stacked parallel beta-sheets; a fold which is distinct from the three known fish AFP structures. The ice-binding side contains 9 of the 14 surface-accessible threonines organized in a regular array of TXT motifs that match the ice lattice on both prism and basal planes. In support of this model, ice crystal morphology and ice-etching experiments are consistent with AFP binding to both of these planes and thus may explain the greater activity of the spruce budworm antifreeze.

Beta-helix structure and ice-binding properties of a hyperactive antifreeze protein from an insect.,Graether SP, Kuiper MJ, Gagne SM, Walker VK, Jia Z, Sykes BD, Davies PL Nature. 2000 Jul 20;406(6793):325-8. PMID:10917537^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Graether SP, Kuiper MJ, Gagne SM, Walker VK, Jia Z, Sykes BD, Davies PL. Beta-helix structure and ice-binding properties of a hyperactive antifreeze protein from an insect. Nature. 2000 Jul 20;406(6793):325-8. PMID:10917537 doi:10.1038/35018610

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1eww"

@@ Line 3: / Line 3: @@
 <StructureSection load='1eww' size='340' side='right'caption='[[1eww]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Archips_fumiferana Archips fumiferana]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EWW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archips_fumiferana Archips fumiferana]. The December 2009 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EWW FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eww OCA], [http://pdbe.org/1eww PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eww RCSB], [http://www.ebi.ac.uk/pdbsum/1eww PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1eww ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eww OCA], [https://pdbe.org/1eww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eww RCSB], [https://www.ebi.ac.uk/pdbsum/1eww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eww ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==

1eww

From Proteopedia

Revision as of 11:08, 28 July 2021

SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEGREES CELSIUS

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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