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1gpt

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Revision as of 11:27, 28 July 2021

SOLUTION STRUCTURE OF GAMMA 1-H AND GAMMA 1-P THIONINS FROM BARLEY AND WHEAT ENDOSPERM DETERMINED BY 1H-NMR: A STRUCTURAL MOTIF COMMON TO TOXIC ARTHROPOD PROTEINS

Structural highlights

1gpt is a 1 chain structure with sequence from Barley. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DEF1_HORVU] Inhibits protein translation in cell-free systems.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The complete assignment of the proton NMR spectra of the homologous gamma 1-hordothionin and gamma 1-purothionin (47 amino acids, 4 disulfide bridges) from barley and wheat, respectively, has been performed by two-dimensional sequence-specific methods. A total of 299 proton-proton distance constraints for gamma 1-H and 285 for gamma 1-P derived from NOESY spectra have been used to calculate the three-dimensional solution structures. Initial structures have been generated by distance geometry methods and further refined by dynamical simulated annealing calculations. Both proteins show identical secondary and tertiary structure with a well-defined triple-stranded antiparallel beta-sheet (residues 1-6, 31-34, and 39-47), an alpha-helix (residues 16-28), and the corresponding connecting loops. Three disulfide bridges are located in the hydrophobic core holding together the alpha-helix and the beta-sheet and forming a cysteine-stabilized alpha-helical (CSH) motif. Moreover, a clustering of positive charges is observed on the face of the beta-sheet opposite to the helix. The three-dimensional structures of the gamma-thionins differ remarkably from plant alpha- and beta-thionins and crambin. However, they show a higher structural analogy with scorpion toxins and insect defensins which also present the CSH motif.

Solution structure of gamma 1-H and gamma 1-P thionins from barley and wheat endosperm determined by 1H-NMR: a structural motif common to toxic arthropod proteins.,Bruix M, Jimenez MA, Santoro J, Gonzalez C, Colilla FJ, Mendez E, Rico M Biochemistry. 1993 Jan 19;32(2):715-24. PMID:8380707^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bruix M, Jimenez MA, Santoro J, Gonzalez C, Colilla FJ, Mendez E, Rico M. Solution structure of gamma 1-H and gamma 1-P thionins from barley and wheat endosperm determined by 1H-NMR: a structural motif common to toxic arthropod proteins. Biochemistry. 1993 Jan 19;32(2):715-24. PMID:8380707

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1gpt"

@@ Line 3: / Line 3: @@
 <StructureSection load='1gpt' size='340' side='right'caption='[[1gpt]], [[NMR_Ensembles_of_Models | 8 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1gpt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Barley Barley]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GPT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1gpt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Barley Barley]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GPT FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpt OCA], [http://pdbe.org/1gpt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gpt RCSB], [http://www.ebi.ac.uk/pdbsum/1gpt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gpt ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpt OCA], [https://pdbe.org/1gpt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gpt RCSB], [https://www.ebi.ac.uk/pdbsum/1gpt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gpt ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/DEF1_HORVU DEF1_HORVU]] Inhibits protein translation in cell-free systems.
+[[https://www.uniprot.org/uniprot/DEF1_HORVU DEF1_HORVU]] Inhibits protein translation in cell-free systems.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

1gpt

From Proteopedia

Revision as of 11:27, 28 July 2021

SOLUTION STRUCTURE OF GAMMA 1-H AND GAMMA 1-P THIONINS FROM BARLEY AND WHEAT ENDOSPERM DETERMINED BY 1H-NMR: A STRUCTURAL MOTIF COMMON TO TOXIC ARTHROPOD PROTEINS

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

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Proteopedia Page Contributors and Editors (what is this?)

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