1kqs

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[[Image:1kqs.gif|left|200px]]
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{{Seed}}
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[[Image:1kqs.png|left|200px]]
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{{STRUCTURE_1kqs| PDB=1kqs | SCENE= }}
{{STRUCTURE_1kqs| PDB=1kqs | SCENE= }}
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'''The Haloarcula marismortui 50S Complexed with a Pretranslocational Intermediate in Protein Synthesis'''
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===The Haloarcula marismortui 50S Complexed with a Pretranslocational Intermediate in Protein Synthesis===
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==Overview==
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The large ribosomal subunit catalyzes peptide bond formation during protein synthesis. Its peptidyl transferase activity has often been studied using a 'fragment assay' that depends on high concentrations of methanol or ethanol. Here we describe a version of this assay that does not require alcohol and use it to show, both crystallographically and biochemically, that crystals of the large ribosomal subunits from Haloarcula marismortui are enzymatically active. Addition of these crystals to solutions containing substrates results in formation of products, which ceases when crystals are removed. When substrates are diffused into large subunit crystals, the subsequent structure shows that products have formed. The CC-puromycin-peptide product is found bound to the A-site and the deacylated CCA is bound to the P-site, with its 3prime prime or minute OH near N3 A2486 (Escherichia coli A2451). Thus, this structure represents a state that occurs after peptide bond formation but before the hybrid state of protein synthesis.
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The line below this paragraph, {{ABSTRACT_PUBMED_11828326}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11828326 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11828326}}
==About this Structure==
==About this Structure==
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[[Category: Ribosome assembly]]
[[Category: Ribosome assembly]]
[[Category: Rna-rna]]
[[Category: Rna-rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:53:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:48:05 2008''

Revision as of 07:48, 2 July 2008

Image:1kqs.png

Template:STRUCTURE 1kqs

The Haloarcula marismortui 50S Complexed with a Pretranslocational Intermediate in Protein Synthesis

Template:ABSTRACT PUBMED 11828326

About this Structure

1KQS is a Protein complex structure of sequences from Haloarcula marismortui. Full crystallographic information is available from OCA.

Reference

A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits., Schmeing TM, Seila AC, Hansen JL, Freeborn B, Soukup JK, Scaringe SA, Strobel SA, Moore PB, Steitz TA, Nat Struct Biol. 2002 Mar;9(3):225-30. PMID:11828326

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