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1pek

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{{STRUCTURE_1pek| PDB=1pek | SCENE= }}
{{STRUCTURE_1pek| PDB=1pek | SCENE= }}
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'''STRUCTURE OF THE COMPLEX OF PROTEINASE K WITH A SUBSTRATE-ANALOGUE HEXA-PEPTIDE INHIBITOR AT 2.2 ANGSTROMS RESOLUTION'''
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===STRUCTURE OF THE COMPLEX OF PROTEINASE K WITH A SUBSTRATE-ANALOGUE HEXA-PEPTIDE INHIBITOR AT 2.2 ANGSTROMS RESOLUTION===
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==Overview==
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The crystal structure of a transition state/product complex formed by the interaction between proteinase K and the substrate analogue N-Ac-L-Pro-L-Ala-L-Pro-L-Phe-D-Ala-L-Ala-NH2 has been determined at a resolution of 2.2 A and refined to an R-factor of 0.165 for 12,725 reflections. The inhibitor forms a stable complex through a series of hydrogen bonds with protein atoms and water molecules. The inhibitor is hydrolyzed between Phe 4I and D-Ala5I (I indicates inhibitor). The two fragments are separated by a distance of 3.07 A between the carbonyl carbon and the main chain nitrogen. Both fragments remain bound to the protein. The N-terminal fragment occupies subsites S5 to S1, whereas the C-terminal part is bound in S1' and S2', the first time that electron density for a substrate analogue has been observed in the P1' and P2' sites of a subtilisin-like enzyme. The flexible segments of the substrate recognition sites Gly100-Tyr104 and Ser132-Gly136 move appreciably to accommodate the inhibitor. Biochemical results indicate an inhibition by this specifically designed peptide of 95%.
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(as it appears on PubMed at http://www.pubmed.gov), where 8340410 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8340410}}
==About this Structure==
==About this Structure==
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[[Category: Wilson, K S.]]
[[Category: Wilson, K S.]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:55:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:41:43 2008''

Revision as of 16:41, 28 July 2008

Image:1pek.png

Template:STRUCTURE 1pek

STRUCTURE OF THE COMPLEX OF PROTEINASE K WITH A SUBSTRATE-ANALOGUE HEXA-PEPTIDE INHIBITOR AT 2.2 ANGSTROMS RESOLUTION

Template:ABSTRACT PUBMED 8340410

About this Structure

1PEK is a Single protein structure. Full crystallographic information is available from OCA.

Reference

Structure of the complex of proteinase K with a substrate analogue hexapeptide inhibitor at 2.2-A resolution., Betzel C, Singh TP, Visanji M, Peters K, Fittkau S, Saenger W, Wilson KS, J Biol Chem. 1993 Jul 25;268(21):15854-8. PMID:8340410

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