|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6mab' size='340' side='right'caption='[[6mab]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='6mab' size='340' side='right'caption='[[6mab]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6mab]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlt2 Chlt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MAB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MAB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6mab]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_trachomatis_434/Bu Chlamydia trachomatis 434/Bu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MAB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MAB FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rbsU, CTL0851 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=471472 CHLT2])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mab FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mab OCA], [http://pdbe.org/6mab PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mab RCSB], [http://www.ebi.ac.uk/pdbsum/6mab PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mab ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mab FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mab OCA], [https://pdbe.org/6mab PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mab RCSB], [https://www.ebi.ac.uk/pdbsum/6mab PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mab ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A0H3MDU0_CHLT2 A0A0H3MDU0_CHLT2] |
| - | Chlamydia trachomatis are obligate intracellular bacteria that undergo dynamic morphologic and physiologic conversions upon gaining access to a eukaryotic cell. These conversions likely require the detection of key environmental conditions and regulation of metabolic activity. Chlamydia encodes homologs to proteins in the Rsb phosphoregulatory partner-switch pathway, best described in Bacillus subtilis. ORF CT588 has strong sequence similarity to RsbU cytoplasmic phosphatase domain but also contains a unique periplasmic sensor domain that is expected to control phosphatase activity. A 1.7 A crystal structure of the periplasmic domain of the RsbU protein from C. trachomatis (PDB 6MAB) displays close structural similarity to DctB from Vibrio and Sinorhizobium. DctB has been shown both structurally and functionally to specifically bind to the TCA cycle intermediate succinate. Surface plasmon resonance and differential scanning fluorimetry of TCA intermediates and potential metabolites from a virtual screen of RsbU revealed that alpha-ketoglutarate, malate, and oxaloacetate bound to the RsbU periplasmic domain. Substitutions in the putative binding site resulted in reduced binding capabilities. A RsbU-null mutant showed severe growth defects which could be restored through genetic complementation. Chemical inhibition of ATP synthesis by oxidative phosphorylation phenocopied the growth defect observed in the RsbU null strain. Altogether, these data support a model with the Rsb system responding differentially to TCA cycle intermediates to regulate metabolism and key differentiation processes.
| + | |
| - | | + | |
| - | Structural and ligand binding analyses of the periplasmic sensor domain of RsbU in Chlamydia trachomatis supports role in TCA cycle regulation.,Soules KR, Dmitriev A, LaBrie SD, Dimond ZE, May BH, Johnson DK, Zhang Y, Battaile KP, Lovell S, Hefty PS Mol Microbiol. 2019 Oct 21. doi: 10.1111/mmi.14401. PMID:31637787<ref>PMID:31637787</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6mab" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chlt2]] | + | [[Category: Chlamydia trachomatis 434/Bu]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Battaile, K P]] | + | [[Category: Battaile KP]] |
| - | [[Category: Dmitriev, A]] | + | [[Category: Dmitriev A]] |
| - | [[Category: Hefty, P S]] | + | [[Category: Hefty PS]] |
| - | [[Category: Lovell, S]] | + | [[Category: Lovell S]] |
| - | [[Category: Soules, K]] | + | [[Category: Soules K]] |
| - | [[Category: Chlamydia trachomati]]
| + | |
| - | [[Category: Periplasmic sensor domain]]
| + | |
| - | [[Category: Rsbu]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| - | [[Category: Tca cycle intermediate]]
| + | |
