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| - | | + | #REDIRECT [[6sct]] This PDB entry is obsolete and replaced by 6sct |
| - | ==Cryo-EM structure of the consensus triskelion hub of the clathrin coat complex==
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| - | <StructureSection load='6sbz' size='340' side='right'caption='[[6sbz]], [[Resolution|resolution]] 4.69Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[6sbz]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SBZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SBZ FirstGlance]. <br>
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| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CLTC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 PIG]), CLTB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 PIG])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sbz OCA], [http://pdbe.org/6sbz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sbz RCSB], [http://www.ebi.ac.uk/pdbsum/6sbz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sbz ProSAT]</span></td></tr>
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| - | </table>
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| - | == Function ==
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| - | [[http://www.uniprot.org/uniprot/C0MHR2_PIG C0MHR2_PIG]] Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.[PIRNR:PIRNR002290] [[http://www.uniprot.org/uniprot/F1S398_PIG F1S398_PIG]] Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.[RuleBase:RU363137]
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Clathrin forms diverse lattice and cage structures that change size and shape rapidly in response to the needs of eukaryotic cells during clathrin-mediated endocytosis and intracellular trafficking. We present the cryo-EM structure and molecular model of assembled porcine clathrin, providing insights into interactions that stabilize key elements of the clathrin lattice, namely, between adjacent heavy chains, at the light chain-heavy chain interface and within the trimerization domain. Furthermore, we report cryo-EM maps for five different clathrin cage architectures. Fitting structural models to three of these maps shows that their assembly requires only a limited range of triskelion leg conformations, yet inherent flexibility is required to maintain contacts. Analysis of the protein-protein interfaces shows remarkable conservation of contact sites despite architectural variation. These data reveal a universal mode of clathrin assembly that allows variable cage architecture and adaptation of coated vesicle size and shape during clathrin-mediated vesicular trafficking or endocytosis.
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| - | Cryo-EM of multiple cage architectures reveals a universal mode of clathrin self-assembly.,Morris KL, Jones JR, Halebian M, Wu S, Baker M, Armache JP, Avila Ibarra A, Sessions RB, Cameron AD, Cheng Y, Smith CJ Nat Struct Mol Biol. 2019 Oct;26(10):890-898. doi: 10.1038/s41594-019-0292-0., Epub 2019 Oct 3. PMID:31582853<ref>PMID:31582853</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 6sbz" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Large Structures]]
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| - | [[Category: Pig]]
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| - | [[Category: Cameron, A D]]
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| - | [[Category: Morris, K L]]
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| - | [[Category: Sessions, R]]
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| - | [[Category: Smith, C J]]
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| - | [[Category: Clathrin]]
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| - | [[Category: Coat protein]]
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| - | [[Category: Endocytosis]]
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| - | [[Category: Trafficking]]
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| - | [[Category: Transport protein]]
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