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== Function ==
== Function ==
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Leadzyme is a relatively small catalytic RNA that uses a unique two-step reaction to cleave within the active site <ref name= "blue">PMID:25962038</ref> . In the presence of Pb^2+, it catalyzes the cleavage of the C6-C7 phosphodiester bond <ref name= red/> . This is accomplished via nucleophilic attack <ref name=red/> . The resulting in a 2', 3'-cyclic phosphate and a 5'-hydroxyl terminus <ref name= red/> . The nuclear magnetic resonance spectrospcopy (NMR) and crystal structure of leadzyme has been used to try to betterr understand the cleavage reaction process <ref name=red/> . However, neither data sets revealed the proposed in-line alignment for attack by the 2'-OH nucleophilic group as described experimentally <ref name=blue> . Therefore: it is hypothesized that the structures represent ground states of leadzyme and are not catalytically active <ref name=blue/> .
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Leadzyme is a relatively small catalytic RNA that uses a unique two-step reaction to cleave within the active site <ref name= "blue">PMID:25962038</ref> . In the presence of Pb^2+, it catalyzes the cleavage of the C6-C7 phosphodiester bond <ref name= red/> . This is accomplished via nucleophilic attack <ref name=red/> . The resulting in a 2', 3'-cyclic phosphate and a 5'-hydroxyl terminus <ref name= red/> . The nuclear magnetic resonance spectrospcopy (NMR) and crystal structure of leadzyme has been used to try to betterr understand the cleavage reaction process <ref name=red/> . However, neither data sets revealed the proposed in-line alignment for attack by the 2'-OH nucleophilic group as described experimentally <ref name=blue> . Therefore: it is hypothesized that the structures represent ground states of leadzyme and are not catalytically active <ref name=blue/> .
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== Disease ==
== Disease ==

Revision as of 16:26, 18 November 2019

This Sandbox is Reserved from September 14, 2021, through May 31, 2022, for use in the class Introduction to Biochemistry taught by User:John Means at the University of Rio Grande, Rio Grande, OH, USA. This reservation includes 5 reserved sandboxes (Sandbox Reserved 1590 through Sandbox Reserved 1594).
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Leadzyme Structure

Leadzyme Structure

Drag the structure with the mouse to rotate

References

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Hoogstraten CG, Legault P, Pardi A. NMR solution structure of the lead-dependent ribozyme: evidence for dynamics in RNA catalysis. J Mol Biol. 1998 Nov 27;284(2):337-50. PMID:9813122 doi:S0022-2836(98)92182-9
  2. ↑ 2.0 2.1 Qi X, Xia T. Structure, dynamics, and mechanism of the lead-dependent ribozyme. Biomol Concepts. 2011 Aug 1;2(4):305-14. doi: 10.1515/bmc.2011.029. PMID:25962038 doi:http://dx.doi.org/10.1515/bmc.2011.029
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