Sandbox Reserved 1586
From Proteopedia
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== History == | == History == | ||
| - | + | Leadzyme was discovered by Uhlenbeck and co-worked in 1992, while they were searching for RNAs that cleaved in the presence of lead <ref name="red">PMID:9813122</ref> . The discovery was by ''in vitro'' selection, which allows for an isolation and amplification of selected functional molecules. This method has been key in the discovery of numerous RNA and DNA catalysis. Leadzyme, or lead-dependent ribozyme, is among the smallest known catalytic RNAs <ref name=red/> . | |
== Function == | == Function == | ||
| - | Leadzyme is a relatively small catalytic RNA that uses a unique two-step reaction to cleave within the active site <ref name= "blue">PMID:25962038</ref> . In the presence of Pb^2+, it catalyzes the cleavage of the C6-C7 phosphodiester bond <ref name= red/> . This is accomplished via nucleophilic attack <ref name=red/> . The resulting in a 2', 3'-cyclic phosphate and a 5'-hydroxyl terminus <ref name= red/> . The nuclear magnetic resonance spectrospcopy (NMR) and crystal structure of leadzyme has been used to try to betterr understand the cleavage reaction process <ref name=red/> . However, neither data sets revealed the proposed in-line alignment for attack by the 2'-OH nucleophilic group as described experimentally <ref name=blue> . Therefore: it is hypothesized that the structures represent ground states of leadzyme and are not catalytically active <ref name=blue/> . | + | Leadzyme is a relatively small catalytic RNA that uses a unique two-step reaction to cleave within the active site <ref name= "blue">PMID:25962038</ref> . |
| + | In the presence of Pb^2+, it catalyzes the cleavage of the C6-C7 phosphodiester bond <ref name= red/> . This is accomplished via nucleophilic attack <ref name=red/> . The resulting in a 2', 3'-cyclic phosphate and a 5'-hydroxyl terminus <ref name= red/> . The nuclear magnetic resonance spectrospcopy (NMR) and crystal structure of leadzyme has been used to try to betterr understand the cleavage reaction process <ref name=red/> . However, neither data sets revealed the proposed in-line alignment for attack by the 2'-OH nucleophilic group as described experimentally <ref name=blue> . Therefore: it is hypothesized that the structures represent ground states of leadzyme and are not catalytically active <ref name=blue/> . | ||
== Disease == | == Disease == | ||
Revision as of 16:27, 18 November 2019
| This Sandbox is Reserved from September 14, 2021, through May 31, 2022, for use in the class Introduction to Biochemistry taught by User:John Means at the University of Rio Grande, Rio Grande, OH, USA. This reservation includes 5 reserved sandboxes (Sandbox Reserved 1590 through Sandbox Reserved 1594). |
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Leadzyme Structure
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References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Hoogstraten CG, Legault P, Pardi A. NMR solution structure of the lead-dependent ribozyme: evidence for dynamics in RNA catalysis. J Mol Biol. 1998 Nov 27;284(2):337-50. PMID:9813122 doi:S0022-2836(98)92182-9
- ↑ 2.0 2.1 Qi X, Xia T. Structure, dynamics, and mechanism of the lead-dependent ribozyme. Biomol Concepts. 2011 Aug 1;2(4):305-14. doi: 10.1515/bmc.2011.029. PMID:25962038 doi:http://dx.doi.org/10.1515/bmc.2011.029
