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| | <StructureSection load='5hzs' size='340' side='right'caption='[[5hzs]], [[Resolution|resolution]] 2.17Å' scene=''> | | <StructureSection load='5hzs' size='340' side='right'caption='[[5hzs]], [[Resolution|resolution]] 2.17Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5hzs]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HZS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HZS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5hzs]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinophyllia_sp._SC22 Echinophyllia sp. SC22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HZS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HZS FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.17Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYS:[(4Z)-2-(1-AMINO-2-HYDROXYETHYL)-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYS</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GYS:[(4Z)-2-(1-AMINO-2-HYDROXYETHYL)-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYS</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2gx2|2gx2]], [[5hzu|5hzu]], [[5hzt|5hzt]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hzs OCA], [https://pdbe.org/5hzs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hzs RCSB], [https://www.ebi.ac.uk/pdbsum/5hzs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hzs ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dronpa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301887 Echinophyllia sp. SC22])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hzs OCA], [http://pdbe.org/5hzs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hzs RCSB], [http://www.ebi.ac.uk/pdbsum/5hzs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hzs ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q5TLG6_9CNID Q5TLG6_9CNID] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Echinophyllia sp. sc22]] | + | [[Category: Echinophyllia sp. SC22]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hwang, K Y]] | + | [[Category: Hwang KY]] |
| - | [[Category: Nam, K H]] | + | [[Category: Nam KH]] |
| - | [[Category: Fluorescent protein]]
| + | |
| - | [[Category: Luminescent protein]]
| + | |
| Structural highlights
Function
Q5TLG6_9CNID
Publication Abstract from PubMed
Many fluorescent proteins (FPs) show fluorescence quenching by specific metal ions, which can be applied towards metal biosensor development. In this study, we investigated the significant fluorescence quenching of Dronpa by Co(2+) and Cu(2+) ions. Crystal structures of Co(2+) -, Ni(2+) - and Cu(2+) -bound Dronpa revealed previously unseen, unique, metal-binding sites for fluorescence quenching. These metal ions commonly interact with surface-exposed histidine residues (His194-His210 and His210-His212), and interact indirectly with chromophores. Structural analysis of the Co(2+) - and Cu(2+) - binding sites of Dronpa provides insight into FP-based metal biosensor engineering.
Crystal structures of Dronpa complexed with quenchable metal ions provide insight into metal biosensor development.,Kim IJ, Kim S, Park J, Eom I, Kim S, Kim JH, Ha SC, Kim YG, Hwang KY, Nam KH FEBS Lett. 2016 Sep;590(17):2982-90. doi: 10.1002/1873-3468.12316. Epub 2016 Jul , 30. PMID:27433793[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kim IJ, Kim S, Park J, Eom I, Kim S, Kim JH, Ha SC, Kim YG, Hwang KY, Nam KH. Crystal structures of Dronpa complexed with quenchable metal ions provide insight into metal biosensor development. FEBS Lett. 2016 Sep;590(17):2982-90. doi: 10.1002/1873-3468.12316. Epub 2016 Jul , 30. PMID:27433793 doi:http://dx.doi.org/10.1002/1873-3468.12316
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