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5t51

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Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly

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 <StructureSection load='5t51' size='340' side='right'caption='[[5t51]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5t51]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_sphaerica Candida sphaerica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T51 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5t51]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T51 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2007&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KLLA0_F02343g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica]), KLLA0_E05809g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t51 OCA], [http://pdbe.org/5t51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t51 RCSB], [http://www.ebi.ac.uk/pdbsum/5t51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t51 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t51 OCA], [https://pdbe.org/5t51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t51 RCSB], [https://www.ebi.ac.uk/pdbsum/5t51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t51 ProSAT]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/Q6CLK3_KLULA Q6CLK3_KLULA]
-Kinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein subassemblies. In budding yeast, the heterotetrameric MIND complex (Mtw1, Nnf1, Nsl1, Dsn1), ortholog of the metazoan Mis12 complex, joins the centromere-proximal components, Mif2 and COMA, with the principal microtubule-binding component, the Ndc80 complex (Ndc80C). We report the crystal structure of Kluyveromyces lactis MIND and examine its partner interactions, to understand the connection from a centromeric nucleosome to a much larger microtubule. MIND resembles an elongated, asymmetric Y; two globular heads project from a coiled-coil shaft. An N-terminal extension of Dsn1 from one head regulates interactions of the other head, blocking binding of Mif2 and COMA. Dsn1 phosphorylation by Ipl1/Aurora B relieves this autoinhibition, enabling MIND to join an assembling kinetochore. A C-terminal extension of Dsn1 recruits Ndc80C to the opposite end of the shaft. The structure and properties of MIND show how it integrates phospho-regulatory inputs for kinetochore assembly and disassembly.
-Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly.,Dimitrova YN, Jenni S, Valverde R, Khin Y, Harrison SC Cell. 2016 Nov 3;167(4):1014-1027.e12. doi: 10.1016/j.cell.2016.10.011. Epub 2016, Oct 27. PMID:27881300<ref>PMID:27881300</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 5t51" style="background-color:#fffaf0;"></div>
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Candida sphaerica]]
+[[Category: Kluyveromyces lactis NRRL Y-1140]]
 [[Category: Large Structures]]
-[[Category: Dimitrova, Y]]
+[[Category: Dimitrova Y]]
-[[Category: Harrison, S C]]
+[[Category: Harrison SC]]
-[[Category: Jenni, S]]
+[[Category: Jenni S]]
-[[Category: Khin, Y]]
+[[Category: Khin Y]]
-[[Category: Valverde, R]]
+[[Category: Valverde R]]
-[[Category: Cell cycle]]
-[[Category: Chromosome]]
-[[Category: Complex]]
-[[Category: Kinetochore]]
-[[Category: Mind]]
-[[Category: Mis12]]
-[[Category: Segregation]]

5t51

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Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly

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