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| | <StructureSection load='5w1h' size='340' side='right'caption='[[5w1h]], [[Resolution|resolution]] 1.99Å' scene=''> | | <StructureSection load='5w1h' size='340' side='right'caption='[[5w1h]], [[Resolution|resolution]] 1.99Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5w1h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lachnospiraceae_bacterium Lachnospiraceae bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W1H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W1H FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5w1h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lachnospiraceae_bacterium Lachnospiraceae bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W1H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W1H FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5w1i|5w1i]], [[5wlh|5wlh]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NK4A179 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1898203 Lachnospiraceae bacterium])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w1h OCA], [https://pdbe.org/5w1h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w1h RCSB], [https://www.ebi.ac.uk/pdbsum/5w1h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w1h ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w1h OCA], [http://pdbe.org/5w1h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w1h RCSB], [http://www.ebi.ac.uk/pdbsum/5w1h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w1h ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A2D0TCG9_9FIRM A0A2D0TCG9_9FIRM] |
| - | CRISPR adaptive immune systems protect bacteria from infections by deploying CRISPR RNA (crRNA)-guided enzymes to recognize and cut foreign nucleic acids. Type VI-A CRISPR-Cas systems include the Cas13a enzyme, an RNA-activated RNase capable of crRNA processing and single-stranded RNA degradation upon target-transcript binding. Here we present the 2.0-A resolution crystal structure of a crRNA-bound Lachnospiraceae bacterium Cas13a (LbaCas13a), representing a recently discovered Cas13a enzyme subtype. This structure and accompanying biochemical experiments define the Cas13a catalytic residues that are directly responsible for crRNA maturation. In addition, the orientation of the foreign-derived target-RNA-specifying sequence in the protein interior explains the conformational gating of Cas13a nuclease activation. These results describe how Cas13a enzymes generate functional crRNAs and how catalytic activity is blocked before target-RNA recognition, with implications for both bacterial immunity and diagnostic applications.
| + | |
| - | | + | |
| - | Guide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme.,Knott GJ, East-Seletsky A, Cofsky JC, Holton JM, Charles E, O'Connell MR, Doudna JA Nat Struct Mol Biol. 2017 Sep 11. doi: 10.1038/nsmb.3466. PMID:28892041<ref>PMID:28892041</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5w1h" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Lachnospiraceae bacterium]] | | [[Category: Lachnospiraceae bacterium]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Doudna, J A]] | + | [[Category: Doudna JA]] |
| - | [[Category: Knott, G J]] | + | [[Category: Knott GJ]] |
| - | [[Category: Nuclease rna binding protein]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Rna binding protein-rna complex]]
| + | |
