| Structural highlights
Function
[GBRG2_RAT] GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. [GBRB1_RAT] Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.[1] [2] [GBRA1_RAT] Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.[3] [4] [5]
Publication Abstract from PubMed
Fast inhibitory neurotransmission in mammalian nervous system is largely mediated by GABAA receptors, chloride-selective members of the superfamily of pentameric Cys-loop receptors. Native GABAA receptors are heteromeric assemblies sensitive to many important drugs, from sedatives to anesthetics and anticonvulsant agents, with mutant forms of GABAA receptors implicated in multiple neurological diseases. Despite profound importance of heteromeric GABAA receptors in neuroscience and medicine, they have proven recalcitrant to structure determination. Here we present the structure of tri-heteromeric alpha1beta1gamma2SEM GABAA receptor in complex with GABA, determined by single particle cryo-EM at 3.1-3.8 A resolution, elucidating molecular principles of receptor assembly and agonist binding. Remarkable N-linked glycosylation on the alpha1 subunit occludes the extracellular vestibule of the ion channel and is poised to modulate receptor assembly and perhaps ion channel gating. Our work provides a pathway to structural studies of heteromeric GABAA receptors and a framework for rational design of novel therapeutic agents.
Cryo-EM structure of the benzodiazepine-sensitive alpha1beta1gamma2S tri-heteromeric GABAA receptor in complex with GABA.,Phulera S, Zhu H, Yu J, Claxton DP, Yoder N, Yoshioka C, Gouaux E Elife. 2018 Jul 25;7. pii: 39383. doi: 10.7554/eLife.39383. PMID:30044221[6]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Saras A, Gisselmann G, Vogt-Eisele AK, Erlkamp KS, Kletke O, Pusch H, Hatt H. Histamine action on vertebrate GABAA receptors: direct channel gating and potentiation of GABA responses. J Biol Chem. 2008 Apr 18;283(16):10470-5. doi: 10.1074/jbc.M709993200. Epub 2008 , Feb 15. PMID:18281286 doi:http://dx.doi.org/10.1074/jbc.M709993200
- ↑ Malherbe P, Draguhn A, Multhaup G, Beyreuther K, Mohler H. GABAA-receptor expressed from rat brain alpha- and beta-subunit cDNAs displays potentiation by benzodiazepine receptor ligands. Brain Res Mol Brain Res. 1990 Aug;8(3):199-208. PMID:1977069
- ↑ Sigel E, Baur R, Kellenberger S, Malherbe P. Point mutations affecting antagonist affinity and agonist dependent gating of GABAA receptor channels. EMBO J. 1992 Jun;11(6):2017-23. PMID:1376242
- ↑ Saras A, Gisselmann G, Vogt-Eisele AK, Erlkamp KS, Kletke O, Pusch H, Hatt H. Histamine action on vertebrate GABAA receptors: direct channel gating and potentiation of GABA responses. J Biol Chem. 2008 Apr 18;283(16):10470-5. doi: 10.1074/jbc.M709993200. Epub 2008 , Feb 15. PMID:18281286 doi:http://dx.doi.org/10.1074/jbc.M709993200
- ↑ Lolait SJ, O'Carroll AM, Kusano K, Muller JM, Brownstein MJ, Mahan LC. Cloning and expression of a novel rat GABAA receptor. FEBS Lett. 1989 Mar 27;246(1-2):145-8. PMID:2540033
- ↑ Phulera S, Zhu H, Yu J, Claxton DP, Yoder N, Yoshioka C, Gouaux E. Cryo-EM structure of the benzodiazepine-sensitive alpha1beta1gamma2S tri-heteromeric GABAA receptor in complex with GABA. Elife. 2018 Jul 25;7. pii: 39383. doi: 10.7554/eLife.39383. PMID:30044221 doi:http://dx.doi.org/10.7554/eLife.39383
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