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They are usually found within non-coding portions of messenger RNAs.<ref name="ghost" /> | They are usually found within non-coding portions of messenger RNAs.<ref name="ghost" /> | ||
| - | Thiamine pyrophosphate is also known as TPP. TPP is essential in all three domains of life, including: bacteria, fungi, and plants. <ref | + | Thiamine pyrophosphate is also known as TPP. TPP is essential in all three domains of life, including: bacteria, fungi, and plants. <ref name="ghost" /> |
| - | They use this specific riboswitch to control genes that are in charge of synthesizing thiamine and any phosphorylated derivatives.<ref | + | They use this specific riboswitch to control genes that are in charge of synthesizing thiamine and any phosphorylated derivatives.<ref name="ghost" /> |
| - | TPP and its riboswitch work together to directly regulate the synthesis of a protein related to TPP. <ref | + | TPP and its riboswitch work together to directly regulate the synthesis of a protein related to TPP. <ref name="ghost" /> |
| - | Thiamine pyrophosphate is the most widely distributed riboswitch of the metabolite-sensing RNA regulatory system. <ref | + | Thiamine pyrophosphate is the most widely distributed riboswitch of the metabolite-sensing RNA regulatory system. <ref name="ghost" /> |
The reason for this is because TPP is a form of vitamin B1, and vitamin B1 takes an essential part in many protein-catalyzed reactions; thus it is used quite often. | The reason for this is because TPP is a form of vitamin B1, and vitamin B1 takes an essential part in many protein-catalyzed reactions; thus it is used quite often. | ||
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== Structural highlights == | == Structural highlights == | ||
| - | TPP riboswitches were one of the first of several classes found to form successful interactions with negatively charged phosphate groups.<ref | + | TPP riboswitches were one of the first of several classes found to form successful interactions with negatively charged phosphate groups.<ref name="ghost" /> |
TPP's pyrophosphate group is bound by a pair of Mg2+ ions, <scene name='82/824626/Tpp/1'>Mg1 and Mg2</scene> . <ref>PMID:16728979</ref> TPP's terminal phosphate group is coordinately bonded to both <scene name='82/824626/Tpp/1'>Mg1 and Mg2</scene>, but the thiazole-linked phosphate is only coordinately bonded to Mg2.<ref>PMID:16728979</ref> | TPP's pyrophosphate group is bound by a pair of Mg2+ ions, <scene name='82/824626/Tpp/1'>Mg1 and Mg2</scene> . <ref>PMID:16728979</ref> TPP's terminal phosphate group is coordinately bonded to both <scene name='82/824626/Tpp/1'>Mg1 and Mg2</scene>, but the thiazole-linked phosphate is only coordinately bonded to Mg2.<ref>PMID:16728979</ref> | ||
| - | Holding Mg1 in place are <scene name='82/824626/Ahhh/2'>G60 and G78</scene> and they can be found within the region that previously was known for pyrophosphate recognition.<ref | + | Holding Mg1 in place are <scene name='82/824626/Ahhh/2'>G60 and G78</scene> and they can be found within the region that previously was known for pyrophosphate recognition.<ref name="ghost" /> Structures of proteins bound to TPP typically position Mg2+, Ca2+, or Mn2+ ions using charged amino acids, in a site equivalent .<ref name="ghost" /> However, the TPP riboswitch is the only riboswitch that contains Mg1 |
Revision as of 17:33, 20 November 2019
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References
- ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
- ↑ Serganov A, Polonskaia A, Phan AT, Breaker RR, Patel DJ. Structural basis for gene regulation by a thiamine pyrophosphate-sensing riboswitch. Nature. 2006 Jun 29;441(7097):1167-71. Epub 2006 May 21. PMID:16728979 doi:http://dx.doi.org/nature04740
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 Serganov A, Polonskaia A, Phan AT, Breaker RR, Patel DJ. Structural basis for gene regulation by a thiamine pyrophosphate-sensing riboswitch. Nature. 2006 Jun 29;441(7097):1167-71. Epub 2006 May 21. PMID:16728979 doi:http://dx.doi.org/nature04740
- ↑ Serganov A, Polonskaia A, Phan AT, Breaker RR, Patel DJ. Structural basis for gene regulation by a thiamine pyrophosphate-sensing riboswitch. Nature. 2006 Jun 29;441(7097):1167-71. Epub 2006 May 21. PMID:16728979 doi:http://dx.doi.org/nature04740
- ↑ Serganov A, Polonskaia A, Phan AT, Breaker RR, Patel DJ. Structural basis for gene regulation by a thiamine pyrophosphate-sensing riboswitch. Nature. 2006 Jun 29;441(7097):1167-71. Epub 2006 May 21. PMID:16728979 doi:http://dx.doi.org/nature04740
- ↑ Serganov A, Polonskaia A, Phan AT, Breaker RR, Patel DJ. Structural basis for gene regulation by a thiamine pyrophosphate-sensing riboswitch. Nature. 2006 Jun 29;441(7097):1167-71. Epub 2006 May 21. PMID:16728979 doi:http://dx.doi.org/nature04740
