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| <StructureSection load='6rje' size='340' side='right'caption='[[6rje]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='6rje' size='340' side='right'caption='[[6rje]], [[Resolution|resolution]] 2.50Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[6rje]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27848 Atcc 27848]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RJE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RJE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6rje]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_simulans Staphylococcus simulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RJE FirstGlance]. <br> |
- | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K5T:(2~{R})-2-[[(2~{S})-2-[[(4~{R})-5-azanyl-4-[[(2~{S})-2-azanylpropanoyl]amino]-5-oxidanylidene-pentanoyl]amino]-6-[2-[2-[2-[2-(2-azanylethanoylamino)ethanoylamino]ethanoylamino]ethanoylamino]ethanoylamino]hexanoyl]amino]propanoic+acid'>K5T</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
- | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6rk4|6rk4]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K5T:(2~{R})-2-[[(2~{S})-2-[[(4~{R})-5-azanyl-4-[[(2~{S})-2-azanylpropanoyl]amino]-5-oxidanylidene-pentanoyl]amino]-6-[2-[2-[2-[2-(2-azanylethanoylamino)ethanoylamino]ethanoylamino]ethanoylamino]ethanoylamino]hexanoyl]amino]propanoic+acid'>K5T</scene></td></tr> |
- | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lss ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1286 ATCC 27848])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rje OCA], [https://pdbe.org/6rje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rje RCSB], [https://www.ebi.ac.uk/pdbsum/6rje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rje ProSAT]</span></td></tr> |
- | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysostaphin Lysostaphin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.75 3.4.24.75] </span></td></tr>
| + | |
- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rje OCA], [http://pdbe.org/6rje PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rje RCSB], [http://www.ebi.ac.uk/pdbsum/6rje PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rje ProSAT]</span></td></tr> | + | |
| </table> | | </table> |
| == Function == | | == Function == |
- | [[http://www.uniprot.org/uniprot/LSTP_STASI LSTP_STASI]] Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan. | + | [https://www.uniprot.org/uniprot/LSTP_STASI LSTP_STASI] Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan. |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
- | [[Category: Atcc 27848]] | |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
- | [[Category: Lysostaphin]] | + | [[Category: Staphylococcus simulans]] |
- | [[Category: Lovering, A L]] | + | [[Category: Lovering AL]] |
- | [[Category: Walters-Morgan, H]] | + | [[Category: Walters-Morgan H]] |
- | [[Category: Peptide binding protein]]
| + | |
- | [[Category: Peptidoglycan hydrolase]]
| + | |
| Structural highlights
Function
LSTP_STASI Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan.
Publication Abstract from PubMed
Lysostaphin is a bacteriolytic enzyme targeting peptidoglycan, the essential component of the bacterial cell envelope. It displays a very potent and specific activity toward staphylococci, including methicillin-resistant Staphylococcus aureus. Lysostaphin causes rapid cell lysis and disrupts biofilms, and is therefore a therapeutic agent of choice to eradicate staphylococcal infections. The C-terminal SH3b domain of lysostaphin recognizes peptidoglycans containing a pentaglycine crossbridge and has been proposed to drive the preferential digestion of staphylococcal cell walls. Here we elucidate the molecular mechanism underpinning recognition of staphylococcal peptidoglycan by the lysostaphin SH3b domain. We show that the pentaglycine crossbridge and the peptide stem are recognized by two independent binding sites located on opposite sides of the SH3b domain, thereby inducing a clustering of SH3b domains. We propose that this unusual binding mechanism allows synergistic and structurally dynamic recognition of S. aureus peptidoglycan and underpins the potent bacteriolytic activity of this enzyme.
Two-site recognition of Staphylococcus aureus peptidoglycan by lysostaphin SH3b.,Gonzalez-Delgado LS, Walters-Morgan H, Salamaga B, Robertson AJ, Hounslow AM, Jagielska E, Sabala I, Williamson MP, Lovering AL, Mesnage S Nat Chem Biol. 2019 Nov 4. pii: 10.1038/s41589-019-0393-4. doi:, 10.1038/s41589-019-0393-4. PMID:31686030[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gonzalez-Delgado LS, Walters-Morgan H, Salamaga B, Robertson AJ, Hounslow AM, Jagielska E, Sabala I, Williamson MP, Lovering AL, Mesnage S. Two-site recognition of Staphylococcus aureus peptidoglycan by lysostaphin SH3b. Nat Chem Biol. 2019 Nov 4. pii: 10.1038/s41589-019-0393-4. doi:, 10.1038/s41589-019-0393-4. PMID:31686030 doi:http://dx.doi.org/10.1038/s41589-019-0393-4
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