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1msw

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Revision as of 06:55, 25 August 2021

Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase

Structural highlights

1msw is a 4 chain structure with sequence from Bpt7. The April 2003 RCSB PDB Molecule of the Month feature on RNA Polymerase by David S. Goodsell is 10.2210/rcsb_pdb/mom_2003_4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Activity:	DNA-directed RNA polymerase, with EC number 2.7.7.6
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RPOL_BPT7] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

To make messenger RNA transcripts, bacteriophage T7 RNA polymerase (T7 RNAP) undergoes a transition from an initiation phase, which only makes short RNA fragments, to a stable elongation phase. We have determined at 2.1 angstrom resolution the crystal structure of a T7 RNAP elongation complex with 30 base pairs of duplex DNA containing a "transcription bubble" interacting with a 17-nucleotide RNA transcript. The transition from an initiation to an elongation complex is accompanied by a major refolding of the amino-terminal 300 residues. This results in loss of the promoter binding site, facilitating promoter clearance, and creates a tunnel that surrounds the RNA transcript after it peels off a seven-base pair heteroduplex. Formation of the exit tunnel explains the enhanced processivity of the elongation complex. Downstream duplex DNA binds to the fingers domain, and its orientation relative to upstream DNA in the initiation complex implies an unwinding that could facilitate formation of the open promoter complex.

Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase.,Yin YW, Steitz TA Science. 2002 Nov 15;298(5597):1387-95. Epub 2002 Sep 19. PMID:12242451^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yin YW, Steitz TA. Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase. Science. 2002 Nov 15;298(5597):1387-95. Epub 2002 Sep 19. PMID:12242451 doi:10.1126/science.1077464

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1msw"

@@ Line 3: / Line 3: @@
 <StructureSection load='1msw' size='340' side='right'caption='[[1msw]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1msw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. The April 2003 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''RNA Polymerase''  by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2003_4 10.2210/rcsb_pdb/mom_2003_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MSW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1msw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpt7 Bpt7]. The April 2003 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''RNA Polymerase''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2003_4 10.2210/rcsb_pdb/mom_2003_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MSW FirstGlance]. <br>
-</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
+</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1msw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msw OCA], [http://pdbe.org/1msw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1msw RCSB], [http://www.ebi.ac.uk/pdbsum/1msw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1msw ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1msw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msw OCA], [https://pdbe.org/1msw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1msw RCSB], [https://www.ebi.ac.uk/pdbsum/1msw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1msw ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/RPOL_BPT7 RPOL_BPT7]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.
+[[https://www.uniprot.org/uniprot/RPOL_BPT7 RPOL_BPT7]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 30: / Line 30: @@
 ==See Also==
-*[[RNA polymerase|RNA polymerase]]
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
 == References ==
 <references/>

1msw

From Proteopedia

Revision as of 06:55, 25 August 2021

Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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