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| | {{STRUCTURE_186d| PDB=186d | SCENE= }} | | {{STRUCTURE_186d| PDB=186d | SCENE= }} |
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| - | '''SOLUTION STRUCTURE OF THE TETRAHYMENA TELOMERIC REPEAT D(T2G4)4 G-TETRAPLEX'''
| + | ===SOLUTION STRUCTURE OF THE TETRAHYMENA TELOMERIC REPEAT D(T2G4)4 G-TETRAPLEX=== |
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| - | ==Overview==
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| - | BACKGROUND: Telomeres in eukaryotic organisms are protein-DNA complexes which are essential for the protection and replication of chromosomal termini. The telomeric DNA of Tetrahymena consists of T2G4 repeats, and models have been previously proposed for the intramolecular folded structure of the d(T2G4)4 sequence based on chemical footprinting and cross-linking data. A high-resolution solution structure of this sequence would allow comparison with the structures of related G-tetraplexes. RESULTS: The solution structure of the Na(+)-stabilized d(T2G4)4 sequence has been determined using a combined NMR-molecular dynamics approach. The sequence folds intramolecularly into a right-handed G-tetraplex containing three stacked G-tetrads connected by linker segments consisting of a G-T-T-G lateral loop, a central T-T-G lateral loop and a T-T segment that spans the groove through a double chain reversal. The latter T-T connectivity aligns adjacent G-G-G segments in parallel and introduces a new G-tetraplex folding topology with unprecedented combinations of strand directionalities and groove widths, as well as guanine syn/anti distributions along individual strands and around individual G-tetrads. CONCLUSIONS: The four repeat Tetrahymena and human G-tetraplexes, which differ by a single guanine for adenine substitution, exhibit strikingly different folding topologies. The observed structural polymorphism establishes that G-tetraplexes can adopt topologies which project distinctly different groove dimensions, G-tetrad base edges and linker segments for recognition by, and interactions with, other nucleic acids and proteins.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_7704525}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 7704525 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_7704525}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=186D OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=186D OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Wang, Y.]] | | [[Category: Wang, Y.]] |
| | [[Category: Dna]] | | [[Category: Dna]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:34:27 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:38:26 2008'' |
Revision as of 12:38, 30 June 2008
Template:STRUCTURE 186d
SOLUTION STRUCTURE OF THE TETRAHYMENA TELOMERIC REPEAT D(T2G4)4 G-TETRAPLEX
Template:ABSTRACT PUBMED 7704525
About this Structure
Full experimental information is available from OCA.
Reference
Solution structure of the Tetrahymena telomeric repeat d(T2G4)4 G-tetraplex., Wang Y, Patel DJ, Structure. 1994 Dec 15;2(12):1141-56. PMID:7704525
Page seeded by OCA on Mon Jun 30 15:38:26 2008
