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4zo2

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Revision as of 07:35, 18 May 2023

AidC, a Dizinc Quorum-Quenching Lactonase

Structural highlights

4zo2 is a 2 chain structure with sequence from Chryseobacterium sp. StRB126. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

I7HB71_9FLAO

Publication Abstract from PubMed

Quorum-quenching catalysts are of interest for potential application as biochemical tools for interrogating interbacterial communication pathways, as antibiofouling agents, and as anti-infective agents in plants and animals. Herein, the structure and function of AidC, an N-acyl-l-homoserine lactone (AHL) lactonase from Chryseobacterium, is characterized. Steady-state kinetics show that zinc-supplemented AidC is the most efficient wild-type quorum-quenching enzymes characterized to date, with a kcat/KM value of approximately 2 x 106 M-1 s-1 for N-heptanoyl-l-homoserine lactone. The enzyme has stricter substrate selectivity and significantly lower KM values (ca. 50 muM for preferred substrates) compared to those of typical AHL lactonases (ca. >1 mM). X-ray crystal structures of AidC alone and with the product N-hexanoyl-l-homoserine were determined at resolutions of 1.09 and 1.67 A, respectively. Each structure displays as a dimer, and dimeric oligiomerization was also observed in solution by size-exclusion chromatography coupled with multiangle light scattering. The structures reveal two atypical features as compared to previously characterized AHL lactonases: a "kinked" alpha-helix that forms part of a closed binding pocket that provides affinity and enforces selectivity for AHL substrates and an active-site His substitution that is usually found in a homologous family of phosphodiesterases. Implications for the catalytic mechanism of AHL lactonases are discussed.

Structural and Biochemical Characterization of AidC, a Quorum-Quenching Lactonase with Atypical Selectivity.,Mascarenhas R, Thomas PW, Wu CX, Nocek BP, Hoang QQ, Liu D, Fast W Biochemistry. 2015 Jul 8. PMID:26115006^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mascarenhas R, Thomas PW, Wu CX, Nocek BP, Hoang QQ, Liu D, Fast W. Structural and Biochemical Characterization of AidC, a Quorum-Quenching Lactonase with Atypical Selectivity. Biochemistry. 2015 Jul 8. PMID:26115006 doi:http://dx.doi.org/10.1021/acs.biochem.5b00499

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4zo2"

@@ Line 3: / Line 3: @@
 <StructureSection load='4zo2' size='340' side='right'caption='[[4zo2]], [[Resolution|resolution]] 1.09&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4zo2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chryseobacterium_sp._strb126 Chryseobacterium sp. strb126]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZO2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZO2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4zo2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chryseobacterium_sp._StRB126 Chryseobacterium sp. StRB126]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZO2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZO2 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zo3|4zo3]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zo2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zo2 OCA], [https://pdbe.org/4zo2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zo2 RCSB], [https://www.ebi.ac.uk/pdbsum/4zo2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zo2 ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aidC, CHSO_3121 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=878220 Chryseobacterium sp. StRB126])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zo2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zo2 OCA], [http://pdbe.org/4zo2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zo2 RCSB], [http://www.ebi.ac.uk/pdbsum/4zo2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zo2 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/I7HB71_9FLAO I7HB71_9FLAO]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Chryseobacterium sp. strb126]]
+[[Category: Chryseobacterium sp. StRB126]]
 [[Category: Large Structures]]
-[[Category: Fast, W]]
+[[Category: Fast W]]
-[[Category: Hoang, Q]]
+[[Category: Hoang Q]]
-[[Category: Liu, D]]
+[[Category: Liu D]]
-[[Category: Mascarenhas, R]]
+[[Category: Mascarenhas R]]
-[[Category: Nocek, B P]]
+[[Category: Nocek BP]]
-[[Category: Thomas, P W]]
+[[Category: Thomas PW]]
-[[Category: Wu, C X]]
+[[Category: Wu C-X]]
-[[Category: Dizinc]]
-[[Category: Hydrolase]]
-[[Category: Lactonase]]
-[[Category: N-acyl-l-homoserine lactone]]
-[[Category: Quorum-quenching]]

4zo2

From Proteopedia

Revision as of 07:35, 18 May 2023

AidC, a Dizinc Quorum-Quenching Lactonase

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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