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4zyr

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Revision as of 07:47, 18 May 2023

Crystal structure of E. coli Lactose permease G46W/G262W bound to p-nitrophenyl alpha-D-galactopyranoside (alpha-NPG)

Structural highlights

4zyr is a 2 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LACY_ECOLI Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).

Publication Abstract from PubMed

The X-ray crystal structure of a conformationally constrained mutant of the Escherichia coli lactose permease (the LacY double-Trp mutant Gly-46-->Trp/Gly-262-->Trp) with bound p-nitrophenyl-alpha-d-galactopyranoside (alpha-NPG), a high-affinity lactose analog, is described. With the exception of Glu-126 (helix IV), side chains Trp-151 (helix V), Glu-269 (helix VIII), Arg-144 (helix V), His-322 (helix X), and Asn-272 (helix VIII) interact directly with the galactopyranosyl ring of alpha-NPG to provide specificity, as indicated by biochemical studies and shown directly by X-ray crystallography. In contrast, Phe-20, Met-23, and Phe-27 (helix I) are within van der Waals distance of the benzyl moiety of the analog and thereby increase binding affinity nonspecifically. Thus, the specificity of LacY for sugar is determined solely by side-chain interactions with the galactopyranosyl ring, whereas affinity is increased by nonspecific hydrophobic interactions with the anomeric substituent.

Structure of LacY with an alpha-substituted galactoside: Connecting the binding site to the protonation site.,Kumar H, Finer-Moore JS, Kaback HR, Stroud RM Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):9004-9. doi:, 10.1073/pnas.1509854112. Epub 2015 Jul 8. PMID:26157133^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kumar H, Finer-Moore JS, Kaback HR, Stroud RM. Structure of LacY with an alpha-substituted galactoside: Connecting the binding site to the protonation site. Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):9004-9. doi:, 10.1073/pnas.1509854112. Epub 2015 Jul 8. PMID:26157133 doi:http://dx.doi.org/10.1073/pnas.1509854112

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4zyr"

@@ Line 3: / Line 3: @@
 <StructureSection load='4zyr' size='340' side='right'caption='[[4zyr]], [[Resolution|resolution]] 3.31&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4zyr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZYR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZYR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4zyr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZYR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZYR FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=9PG:4-NITROPHENYL+ALPHA-D-GALACTOPYRANOSIDE'>9PG</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9PG:4-NITROPHENYL+ALPHA-D-GALACTOPYRANOSIDE'>9PG</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1pv7|1pv7]], [[2v8n|2v8n]], [[2y5y|2y5y]], [[4oaa|4oaa]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zyr OCA], [https://pdbe.org/4zyr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zyr RCSB], [https://www.ebi.ac.uk/pdbsum/4zyr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zyr ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lacY, b0343, JW0334 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zyr OCA], [http://pdbe.org/4zyr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zyr RCSB], [http://www.ebi.ac.uk/pdbsum/4zyr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zyr ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI]] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
+[https://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 27: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Ecoli]]
+[[Category: Escherichia coli K-12]]
 [[Category: Large Structures]]
-[[Category: Finer-Moore, J S]]
+[[Category: Finer-Moore JS]]
-[[Category: Kaback, H R]]
+[[Category: Kaback HR]]
-[[Category: Kumar, H]]
+[[Category: Kumar H]]
-[[Category: Stroud, R M]]
+[[Category: Stroud RM]]
-[[Category: Alpha-helical]]
-[[Category: Membrane protein]]
-[[Category: Transport protein]]
-[[Category: Transporter]]

4zyr

From Proteopedia

Revision as of 07:47, 18 May 2023

Crystal structure of E. coli Lactose permease G46W/G262W bound to p-nitrophenyl alpha-D-galactopyranoside (alpha-NPG)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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