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| | <StructureSection load='5kwd' size='340' side='right'caption='[[5kwd]], [[Resolution|resolution]] 2.75Å' scene=''> | | <StructureSection load='5kwd' size='340' side='right'caption='[[5kwd]], [[Resolution|resolution]] 2.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5kwd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Metallosphaera_sp._mk1 Metallosphaera sp. mk1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KWD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KWD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5kwd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Metallosphaera_yellowstonensis_MK1 Metallosphaera yellowstonensis MK1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KWD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KWD FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MetMK1DRAFT_00016260 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=671065 Metallosphaera sp. MK1])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kwd OCA], [http://pdbe.org/5kwd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kwd RCSB], [http://www.ebi.ac.uk/pdbsum/5kwd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kwd ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kwd OCA], [https://pdbe.org/5kwd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kwd RCSB], [https://www.ebi.ac.uk/pdbsum/5kwd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kwd ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Metallosphaera sp. mk1]] | + | [[Category: Metallosphaera yellowstonensis MK1]] |
| - | [[Category: Baker, D]] | + | [[Category: Baker D]] |
| - | [[Category: Fallas, J A]] | + | [[Category: Fallas JA]] |
| - | [[Category: Sankaran, B]] | + | [[Category: Sankaran B]] |
| - | [[Category: Zwart, P]] | + | [[Category: Zwart P]] |
| - | [[Category: Ankyrin]]
| + | |
| - | [[Category: De novo protein]]
| + | |
| - | [[Category: Designed protein]]
| + | |
| - | [[Category: Homooligomer]]
| + | |
| - | [[Category: Repeat protein]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Self-assembling cyclic protein homo-oligomers play important roles in biology, and the ability to generate custom homo-oligomeric structures could enable new approaches to probe biological function. Here we report a general approach to design cyclic homo-oligomers that employs a new residue-pair-transform method to assess the designability of a protein-protein interface. This method is sufficiently rapid to enable the systematic enumeration of cyclically docked arrangements of a monomer followed by sequence design of the newly formed interfaces. We use this method to design interfaces onto idealized repeat proteins that direct their assembly into complexes that possess cyclic symmetry. Of 96 designs that were characterized experimentally, 21 were found to form stable monodisperse homo-oligomers in solution, and 15 (four homodimers, six homotrimers, six homotetramers and one homopentamer) had solution small-angle X-ray scattering data consistent with the design models. X-ray crystal structures were obtained for five of the designs and each is very close to their corresponding computational model.
Computational design of self-assembling cyclic protein homo-oligomers.,Fallas JA, Ueda G, Sheffler W, Nguyen V, McNamara DE, Sankaran B, Pereira JH, Parmeggiani F, Brunette TJ, Cascio D, Yeates TR, Zwart P, Baker D Nat Chem. 2017 Apr;9(4):353-360. doi: 10.1038/nchem.2673. Epub 2016 Dec 5. PMID:28338692[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fallas JA, Ueda G, Sheffler W, Nguyen V, McNamara DE, Sankaran B, Pereira JH, Parmeggiani F, Brunette TJ, Cascio D, Yeates TR, Zwart P, Baker D. Computational design of self-assembling cyclic protein homo-oligomers. Nat Chem. 2017 Apr;9(4):353-360. doi: 10.1038/nchem.2673. Epub 2016 Dec 5. PMID:28338692 doi:http://dx.doi.org/10.1038/nchem.2673
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