6d6z
From Proteopedia
(Difference between revisions)
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| - | ==Structure of the | + | ==Structure of the malate racemase apoprotein from Thermoanaerobacterium thermosaccharolyticum== |
<StructureSection load='6d6z' size='340' side='right'caption='[[6d6z]], [[Resolution|resolution]] 2.38Å' scene=''> | <StructureSection load='6d6z' size='340' side='right'caption='[[6d6z]], [[Resolution|resolution]] 2.38Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6d6z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermosaccharolyticum Clostridium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D6Z OCA]. For a <b>guided tour on the structure components</b> use [http:// | + | <table><tr><td colspan='2'>[[6d6z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermosaccharolyticum Clostridium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D6Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6D6Z FirstGlance]. <br> |
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tthe_2432 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=580327 Clostridium thermosaccharolyticum])</td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tthe_2432 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=580327 Clostridium thermosaccharolyticum])</td></tr> | ||
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6d6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d6z OCA], [http://pdbe.org/6d6z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d6z RCSB], [http://www.ebi.ac.uk/pdbsum/6d6z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d6z ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Isomerization reactions are fundamental in biology. Lactate racemase, which isomerizes L- and D-lactate, is composed of the LarA protein and a nickel-containing cofactor, the nickel-pincer nucleotide (NPN). In this study, we show that LarA is part of a superfamily containing many different enzymes. We overexpressed and purified 13 lactate racemase homologs, incorporated the NPN cofactor, and assayed the isomerization of different substrates guided by gene context analysis. We discovered two malate racemases, one phenyllactate racemase, one alpha-hydroxyglutarate racemase, two D-gluconate 2-epimerases, and one short-chain aliphatic alpha-hydroxyacid racemase among the tested enzymes. We solved the structure of a malate racemase apoprotein and used it, along with the previously described structures of lactate racemase holoprotein and D-gluconate epimerase apoprotein, to identify key residues involved in substrate binding. This study demonstrates that the NPN cofactor is used by a diverse superfamily of alpha-hydroxyacid racemases and epimerases, widely expanding the scope of NPN-dependent enzymes. | ||
| + | |||
| + | Uncovering a superfamily of nickel-dependent hydroxyacid racemases and epimerases.,Desguin B, Urdiain-Arraiza J, Da Costa M, Fellner M, Hu J, Hausinger RP, Desmet T, Hols P, Soumillion P Sci Rep. 2020 Oct 22;10(1):18123. doi: 10.1038/s41598-020-74802-6. PMID:33093595<ref>PMID:33093595</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6d6z" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 07:28, 4 November 2020
Structure of the malate racemase apoprotein from Thermoanaerobacterium thermosaccharolyticum
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