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| | <StructureSection load='6dyl' size='340' side='right'caption='[[6dyl]], [[Resolution|resolution]] 1.69Å' scene=''> | | <StructureSection load='6dyl' size='340' side='right'caption='[[6dyl]], [[Resolution|resolution]] 1.69Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6dyl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DYL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DYL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6dyl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DYL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DYL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=V:VANADIUM+ION'>V</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.691Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6dyk|6dyk]], [[6dyj|6dyj]], [[6dy8|6dy8]], [[6dy4|6dy4]], [[6dy6|6dy6]], [[6dyh|6dyh]], [[6dyf|6dyf]], [[6dyg|6dyg]], [[6dyd|6dyd]], [[6dye|6dye]], [[6dyc|6dyc]], [[6dyb|6dyb]], [[6dyi|6dyi]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=V:VANADIUM+ION'>V</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cybC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dyl OCA], [https://pdbe.org/6dyl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dyl RCSB], [https://www.ebi.ac.uk/pdbsum/6dyl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dyl ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dyl OCA], [http://pdbe.org/6dyl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dyl RCSB], [http://www.ebi.ac.uk/pdbsum/6dyl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dyl ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/C562_ECOLX C562_ECOLX]] Electron-transport protein of unknown function. | + | [https://www.uniprot.org/uniprot/C562_ECOLX C562_ECOLX] Electron-transport protein of unknown function. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Rittle, J]] | + | [[Category: Rittle J]] |
| - | [[Category: Tezcan, F A]] | + | [[Category: Tezcan FA]] |
| - | [[Category: 4-helix bundle]]
| + | |
| - | [[Category: Designed protein]]
| + | |
| - | [[Category: Metal binding protein]]
| + | |
| Structural highlights
Function
C562_ECOLX Electron-transport protein of unknown function.
Publication Abstract from PubMed
The bottom-up design and construction of functional metalloproteins remains a formidable task in biomolecular design. Although numerous strategies have been used to create new metalloproteins, pre-existing knowledge of the tertiary and quaternary protein structure is often required to generate suitable platforms for robust metal coordination and activity. Here we report an alternative and easily implemented approach (metal active sites by covalent tethering or MASCoT) in which folded protein building blocks are linked by a single disulfide bond to create diverse metal coordination environments within evolutionarily naive protein-protein interfaces. Metalloproteins generated using this strategy uniformly bind a wide array of first-row transition metal ions (Mn(II), Fe(II), Co(II), Ni(II), Cu(II), Zn(II) and vanadyl) with physiologically relevant thermodynamic affinities (dissociation constants ranging from 700 nM for Mn(II) to 50 fM for Cu(II)). MASCoT readily affords coordinatively unsaturated metal centres-including a penta-His-coordinated non-haem Fe site-and well-defined binding pockets that can accommodate modifications and enable coordination of exogenous ligands such as nitric oxide to the interfacial metal centre.
An efficient, step-economical strategy for the design of functional metalloproteins.,Rittle J, Field MJ, Green MT, Tezcan FA Nat Chem. 2019 Feb 18. pii: 10.1038/s41557-019-0218-9. doi:, 10.1038/s41557-019-0218-9. PMID:30778140[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Rittle J, Field MJ, Green MT, Tezcan FA. An efficient, step-economical strategy for the design of functional metalloproteins. Nat Chem. 2019 Feb 18. pii: 10.1038/s41557-019-0218-9. doi:, 10.1038/s41557-019-0218-9. PMID:30778140 doi:http://dx.doi.org/10.1038/s41557-019-0218-9
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