194l

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{{STRUCTURE_194l| PDB=194l | SCENE= }}
{{STRUCTURE_194l| PDB=194l | SCENE= }}
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'''THE 1.40 A STRUCTURE OF SPACEHAB-01 HEN EGG WHITE LYSOZYME'''
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===THE 1.40 A STRUCTURE OF SPACEHAB-01 HEN EGG WHITE LYSOZYME===
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==Overview==
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Crystals of tetragonal hen egg-white lysozyme were grown using Advanced Protein Crystallization Facility (APCF) apparatus under a microgravity environment (SpaceHab-01 mission) and ground control conditions. Crystals were grown from NaCl as a crystallizing agent at pH 4.3. The X-ray diffraction patterns of the best diffracting ground- and space-grown crystals were recorded using synchrotron radiation and an image plate on the W32 beamline at LURE. Both ground- and space-grown crystals showed nearly equivalent maximum resolution of 1.3-1.4 A. Refinements were carried out with the program X-PLOR with final R values of 18.45 and 18.27% for structures from ground- and space- grown crystals, respectively. The two structures are nearly identical with the root-mean-square difference on all protein atoms being 0.13 A. Some residues of the two refined structures show multiple alternative conformations. Two ions were localized into the electron-density maps of the two structures: one chloride ion at the interface between two symmetry-related molecules and one sodium ion stabilizing the loop Ser60-Leu75. The sodium ion is surrounded by six ligands which form a bipyramid around it at distances of 2.2-2.6 A.
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(as it appears on PubMed at http://www.pubmed.gov), where 15299672 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15299672}}
==About this Structure==
==About this Structure==
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[[Category: Ries-Kautt, M.]]
[[Category: Ries-Kautt, M.]]
[[Category: Vaney, M C.]]
[[Category: Vaney, M C.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:40:06 2008''

Revision as of 12:40, 30 June 2008

Image:194l.png

Template:STRUCTURE 194l

THE 1.40 A STRUCTURE OF SPACEHAB-01 HEN EGG WHITE LYSOZYME

Template:ABSTRACT PUBMED 15299672

About this Structure

194L is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

High-resolution structure (1.33 A) of a HEW lysozyme tetragonal crystal grown in the APCF apparatus. Data and structural comparison with a crystal grown under microgravity from SpaceHab-01 mission., Vaney MC, Maignan S, Ries-Kautt M, Ducriux A, Acta Crystallogr D Biol Crystallogr. 1996 May 1;52(Pt 3):505-17. PMID:15299672

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