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| | <StructureSection load='6l33' size='340' side='right'caption='[[6l33]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='6l33' size='340' side='right'caption='[[6l33]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6l33]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L33 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L33 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6l33]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L33 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L33 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mexT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l33 OCA], [https://pdbe.org/6l33 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l33 RCSB], [https://www.ebi.ac.uk/pdbsum/6l33 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l33 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l33 OCA], [http://pdbe.org/6l33 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l33 RCSB], [http://www.ebi.ac.uk/pdbsum/6l33 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l33 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9I0Z0_PSEAE Q9I0Z0_PSEAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6l33" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6l33" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ha, N C]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Kim, S]] | + | [[Category: Ha N-C]] |
| - | [[Category: Antibiotic resistance]]
| + | [[Category: Kim S]] |
| - | [[Category: Lysr-type transcriptional regulator]]
| + | |
| - | [[Category: Regulatory domain]] | + | |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
Q9I0Z0_PSEAE
Publication Abstract from PubMed
The Gram-negative opportunistic pathogen, Pseudomonas aeruginosa, has multiple multidrug efflux pumps. MexT, a LysR-type transcriptional regulator, functions as a transcriptional activator of the MexEF-OprN efflux system. MexT consists of an N-terminal DNA-binding domain and a C-terminal regulatory domain (RD). Little is known regarding MexT ligands and its mechanism of activation. We elucidated the crystal structure of the MexT RD at 2.0 A resolution. The structure comprised two protomer chains in a dimeric arrangement. MexT possessed an arginine-rich region and a hydrophobic patch lined by a variable loop, both of which are putative ligand-binding sites. The three-dimensional structure of MexT provided clues to the interacting ligand structure. A DNase I footprinting assay of full-length MexT identified two MexT-binding sequence in the mexEF-oprN promoter. Our findings enhance the understanding of the regulation of MexT-dependent activation of efflux pumps.
Crystal Structure of the Regulatory Domain of MexT, a Transcriptional Activator of the MexEFOprN Efflux Pump in Pseudomonas aeruginosa.,Kim S, Kim SH, Ahn J, Jo I, Lee ZW, Choi SH, Ha N Mol Cells. 2019 Nov 14. pii: molcells.2019.0168. doi:, 10.14348/molcells.2019.0168. PMID:31722511[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kim S, Kim SH, Ahn J, Jo I, Lee ZW, Choi SH, Ha N. Crystal Structure of the Regulatory Domain of MexT, a Transcriptional Activator of the MexEFOprN Efflux Pump in Pseudomonas aeruginosa. Mol Cells. 2019 Nov 14. pii: molcells.2019.0168. doi:, 10.14348/molcells.2019.0168. PMID:31722511 doi:http://dx.doi.org/10.14348/molcells.2019.0168
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