Sandbox Reserved 1579
From Proteopedia
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==Nitrate Transporter== | ==Nitrate Transporter== | ||
<StructureSection load='4IU9' size='340' side='right' caption='Nitrate Transporter=''> | <StructureSection load='4IU9' size='340' side='right' caption='Nitrate Transporter=''> | ||
| - | Nitrate Transporter is a protein compound found in bacteria, specifically Escherichia coli<ref>PMID:11967075</ref>.This protein compound is a complex made up of NarK and NarU. | + | Nitrate Transporter is a protein compound found in bacteria, specifically Escherichia coli<ref>PMID:11967075</ref>.This protein compound is a complex made up of NarK and NarU. NarU has appeared to be a bigger factor in the function than NarK but both are functionally important. |
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== Function == | == Function == | ||
| - | The Nitrate Transporter complex is active in regulation of nitrogen in the bacteria cell <ref>PMID:23523348</ref>. The complex is expected to provide both nitrate and nitrite transport mechanisms <ref>PMID:11967075</ref>. This transport of Nitrate is under speculation of being different than the typical canonical rocker-switch model<ref>PMID:23523348</ref>. On the NarU portion, | + | The Nitrate Transporter complex is active in regulation of nitrogen in the bacteria cell <ref>PMID:23523348</ref>. The complex is expected to provide both nitrate and nitrite transport mechanisms <ref>PMID:11967075</ref>. This transport of Nitrate is under speculation of being different than the typical canonical rocker-switch model<ref>PMID:23523348</ref>. On the NarU portion, there are four amino acids that have solid electron density and has a functional value in nitrate regulation. NarK does not seem to have the same electron density for an obvious binding site. In NarU, the transport mechanism was severely hindered when these amino acid sites were mutated. And when the amino acids were mutated, the binding site was prevented by the mutation from completing its function. |
== Disease == | == Disease == | ||
No diseases have been linked to the Nitrate transporter protein and any mutation of this protein does not seems to increase or decrease E. coli's growth or activity <ref>PMID:2196029</ref>. | No diseases have been linked to the Nitrate transporter protein and any mutation of this protein does not seems to increase or decrease E. coli's growth or activity <ref>PMID:2196029</ref>. | ||
== Relevance == | == Relevance == | ||
| - | The regulation of nitrate in a | + | The regulation of nitrate in a bacteria cell is necessary in order to maintain homeostasis. |
== Structural highlights == | == Structural highlights == | ||
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| + | The structure of Nitrate transporter does not have many significant sections. NarK is not shown in the picture but the NarU does have amino acids to feature. Specifically Arg87, Arg303, As173, and Tyr261 are four amino acids that have solid electron density and seems to have functional importance. | ||
This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
Revision as of 16:01, 30 November 2019
| This Sandbox is Reserved from September 14, 2021, through May 31, 2022, for use in the class Introduction to Biochemistry taught by User:John Means at the University of Rio Grande, Rio Grande, OH, USA. This reservation includes 5 reserved sandboxes (Sandbox Reserved 1590 through Sandbox Reserved 1594). |
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More help: Help:Editing. For an example of a student Proteopedia page, please see Photosystem II, Tetanospasmin, or Guanine riboswitch. |
Nitrate Transporter
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References
- ↑ Clegg S, Yu F, Griffiths L, Cole JA. The roles of the polytopic membrane proteins NarK, NarU and NirC in Escherichia coli K-12: two nitrate and three nitrite transporters. Mol Microbiol. 2002 Apr;44(1):143-55. doi: 10.1046/j.1365-2958.2002.02858.x. PMID:11967075 doi:http://dx.doi.org/10.1046/j.1365-2958.2002.02858.x
- ↑ Yan H, Huang W, Yan C, Gong X, Jiang S, Zhao Y, Wang J, Shi Y. Structure and mechanism of a nitrate transporter. Cell Rep. 2013 Mar 28;3(3):716-23. doi: 10.1016/j.celrep.2013.03.007. Epub 2013, Mar 21. PMID:23523348 doi:http://dx.doi.org/10.1016/j.celrep.2013.03.007
- ↑ Clegg S, Yu F, Griffiths L, Cole JA. The roles of the polytopic membrane proteins NarK, NarU and NirC in Escherichia coli K-12: two nitrate and three nitrite transporters. Mol Microbiol. 2002 Apr;44(1):143-55. doi: 10.1046/j.1365-2958.2002.02858.x. PMID:11967075 doi:http://dx.doi.org/10.1046/j.1365-2958.2002.02858.x
- ↑ Yan H, Huang W, Yan C, Gong X, Jiang S, Zhao Y, Wang J, Shi Y. Structure and mechanism of a nitrate transporter. Cell Rep. 2013 Mar 28;3(3):716-23. doi: 10.1016/j.celrep.2013.03.007. Epub 2013, Mar 21. PMID:23523348 doi:http://dx.doi.org/10.1016/j.celrep.2013.03.007
- ↑ Brons HJ, Zehnder AJ. Aerobic nitrate and nitrite reduction in continuous cultures of Escherichia coli E4. Arch Microbiol. 1990;153(6):531-6. doi: 10.1007/bf00245261. PMID:2196029 doi:http://dx.doi.org/10.1007/bf00245261
