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4gsp
From Proteopedia
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| - | [[Image:4gsp. | + | [[Image:4gsp.jpg|left|200px]]<br /><applet load="4gsp" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="4gsp" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="4gsp, resolution 1.65Å" /> | caption="4gsp, resolution 1.65Å" /> | ||
'''RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS + 3'-GMP, 7 DAYS'''<br /> | '''RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS + 3'-GMP, 7 DAYS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 4GSP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae] with CA and 3GP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] | + | 4GSP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae] with CA and 3GP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] Known structural/functional Sites: <scene name='pdbsite=BI1:Site'>BI1</scene>, <scene name='pdbsite=CAL:Site'>CAL</scene> and <scene name='pdbsite=CAT:Active Site'>CAT</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=4GSP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 20:44:18 2007'' |
Revision as of 18:34, 18 December 2007
|
RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS + 3'-GMP, 7 DAYS
Overview
Here we present a time-resolved crystallographic analysis of the, hydrolysis of exo (Sp) guanosine 2',3'-cyclophosphorothioate by RNase T1., The use of a slow substrate and fast crystallization methods made it, possible to perform the study with conventional data-collection, techniques. The results support the idea that the hydrolysis reaction, proceeds through a mechanism that is the inverse of the, transesterification reaction. In addition, the structures provide an, explanation for the differential behavior of RNase T1 towards exo- and, endo-cyclic thiophosphates.
About this Structure
4GSP is a Single protein structure of sequence from Aspergillus oryzae with CA and 3GP as ligands. Active as Ribonuclease T(1), with EC number 3.1.27.3 Known structural/functional Sites: , and . Full crystallographic information is available from OCA.
Reference
Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography., Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L, Nat Struct Biol. 1998 Apr;5(4):280-3. PMID:9546218
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