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| - | [[Image:1a0m.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1a0m| PDB=1a0m | SCENE= }} | | {{STRUCTURE_1a0m| PDB=1a0m | SCENE= }} |
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| - | '''1.1 ANGSTROM CRYSTAL STRUCTURE OF A-CONOTOXIN [TYR15]-EPI'''
| + | ===1.1 ANGSTROM CRYSTAL STRUCTURE OF A-CONOTOXIN [TYR15]-EPI=== |
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| - | ==Overview==
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| - | Conotoxins are valuable probes of receptors and ion channels because of their small size and highly selective activity. alpha-Conotoxin EpI, a 16-residue peptide from the mollusk-hunting Conus episcopatus, has the amino acid sequence GCCSDPRCNMNNPDY(SO3H)C-NH2 and appears to be an extremely potent and selective inhibitor of the alpha3beta2 and alpha3beta4 neuronal subtypes of the nicotinic acetylcholine receptor (nAChR). The desulfated form of EpI ([Tyr15]EpI) has a potency and selectivity for the nAChR receptor similar to those of EpI. Here we describe the crystal structure of [Tyr15]EpI solved at a resolution of 1.1 A using SnB. The asymmetric unit has a total of 284 non-hydrogen atoms, making this one of the largest structures solved de novo by direct methods. The [Tyr15]EpI structure brings to six the number of alpha-conotoxin structures that have been determined to date. Four of these, [Tyr15]EpI, PnIA, PnIB, and MII, have an alpha4/7 cysteine framework and are selective for the neuronal subtype of the nAChR. The structure of [Tyr15]EpI has the same backbone fold as the other alpha4/7-conotoxin structures, supporting the notion that this conotoxin cysteine framework and spacing give rise to a conserved fold. The surface charge distribution of [Tyr15]EpI is similar to that of PnIA and PnIB but is likely to be different from that of MII, suggesting that [Tyr15]EpI and MII may have different binding modes for the same receptor subtype.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9708977}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9708977 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9708977}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Crystal structure]] | | [[Category: Crystal structure]] |
| | [[Category: Neurotoxin]] | | [[Category: Neurotoxin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:38:01 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:44:16 2008'' |
Revision as of 12:44, 30 June 2008
Template:STRUCTURE 1a0m
1.1 ANGSTROM CRYSTAL STRUCTURE OF A-CONOTOXIN [TYR15]-EPI
Template:ABSTRACT PUBMED 9708977
About this Structure
1A0M is a Single protein structure of sequence from Conus episcopatus. Full crystallographic information is available from OCA.
Reference
The 1.1 A resolution crystal structure of [Tyr15]EpI, a novel alpha-conotoxin from Conus episcopatus, solved by direct methods., Hu SH, Loughnan M, Miller R, Weeks CM, Blessing RH, Alewood PF, Lewis RJ, Martin JL, Biochemistry. 1998 Aug 18;37(33):11425-33. PMID:9708977
Page seeded by OCA on Mon Jun 30 15:44:16 2008
