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| - | [[Image:1a13.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1a13| PDB=1a13 | SCENE= }} | | {{STRUCTURE_1a13| PDB=1a13 | SCENE= }} |
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| - | '''G PROTEIN-BOUND CONFORMATION OF MASTOPARAN-X, NMR, 14 STRUCTURES'''
| + | ===G PROTEIN-BOUND CONFORMATION OF MASTOPARAN-X, NMR, 14 STRUCTURES=== |
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| - | ==Overview==
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| - | Mastoparans, a family of tetradecapeptides from wasp venom, have been used as convenient low molecular weight models of receptors coupled to GTP-binding regulatory proteins (G proteins) for the understanding of the interaction between G proteins and receptors. Sukumar and Higashijima have analyzed the conformation of mastoparan-X (MP-X) bound to the G protein alpha-subunit using proton two-dimensional transferred nuclear Overhauser effect (TRNOE) spectroscopy [Sukumar, M., and Higashijima, T. (1992) J. Biol. Chem., 267, 21421-21424]. The resultant structure, however, was not well-defined due to severe overlap of peptide proton resonances. To determine the G protein-bound conformation of MP-X in detail, we have analyzed this interaction by heteronuclear multidimensional TRNOE experiments of MP-X uniformly enriched with 15N and/or 13C. By solving the overlap problem, we were able to determine the precise conformation of MP-X bound to Gi1alpha: the peptide adopts an amphiphilic alpha-helix from Trp3 to C-terminal Leu14, and the atomic root-mean-square deviation (rmsd) values in this portion about the averaged coordinates were 0.27 +/- 0.07 A for the backbone atoms (N, Calpha, C') and 0.84 +/- 0.16 A for all heavy atoms. These values are much smaller than the corresponding rmsd values of the structures obtained from the proton 2D TRNOE spectrum alone: 1.70 +/- 0.41 A for the backbone atoms (N, Calpha, C') and 2.84 +/- 0.51 A for all heavy atoms. Our results indicate that the heteronuclear multidimensional TRNOE experiments of peptides uniformly enriched with stable isotopes are a very powerful tool for analyzing the conformation of short peptides bound to large proteins. We will also discuss the structure-activity relationships of mastoparans in activating G proteins on the basis of the precise structure of MP-X bound to Gi1alpha.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9537994}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9537994 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9537994}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 1A13 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vespa_simillima_xanthoptera Vespa simillima xanthoptera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A13 OCA]. | + | 1A13 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vespa_simillima_xanthoptera Vespa simillima xanthoptera]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A13 OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Wakamatsu, K.]] | | [[Category: Wakamatsu, K.]] |
| | [[Category: Mast cell degranulation]] | | [[Category: Mast cell degranulation]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:39:01 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:45:43 2008'' |
Revision as of 12:45, 30 June 2008
Template:STRUCTURE 1a13
G PROTEIN-BOUND CONFORMATION OF MASTOPARAN-X, NMR, 14 STRUCTURES
Template:ABSTRACT PUBMED 9537994
About this Structure
1A13 is a Single protein structure of sequence from Vespa simillima xanthoptera. Full experimental information is available from OCA.
Reference
G protein-bound conformation of mastoparan-X: heteronuclear multidimensional transferred nuclear overhauser effect analysis of peptide uniformly enriched with 13C and 15N., Kusunoki H, Wakamatsu K, Sato K, Miyazawa T, Kohno T, Biochemistry. 1998 Apr 7;37(14):4782-90. PMID:9537994
Page seeded by OCA on Mon Jun 30 15:45:43 2008
