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| | <StructureSection load='5eyb' size='340' side='right'caption='[[5eyb]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='5eyb' size='340' side='right'caption='[[5eyb]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5eyb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EYB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EYB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5eyb]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EYB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EYB FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">reb1, SPBC1198.11c, SPBC660.01c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5eyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eyb OCA], [http://pdbe.org/5eyb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eyb RCSB], [http://www.ebi.ac.uk/pdbsum/5eyb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eyb ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eyb OCA], [https://pdbe.org/5eyb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eyb RCSB], [https://www.ebi.ac.uk/pdbsum/5eyb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eyb ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/REB1_SCHPO REB1_SCHPO]] DNA-binding protein that recognizes sites within both the enhancer and the promoter of rRNA transcription, as well as upstream of many genes transcribed by RNA polymerase II. Has a role in the termination of RNA polymerase I catalyzed transcription.<ref>PMID:9016645</ref> | + | [https://www.uniprot.org/uniprot/REB1_SCHPO REB1_SCHPO] DNA-binding protein that recognizes sites within both the enhancer and the promoter of rRNA transcription, as well as upstream of many genes transcribed by RNA polymerase II. Has a role in the termination of RNA polymerase I catalyzed transcription.<ref>PMID:9016645</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fission yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bastia, D]] | + | [[Category: Schizosaccharomyces pombe 972h-]] |
| - | [[Category: Choudhury, M]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Escalante, C R]] | + | [[Category: Bastia D]] |
| - | [[Category: Jaiswal, R]] | + | [[Category: Choudhury M]] |
| - | [[Category: Santosh, V]] | + | [[Category: Escalante CR]] |
| - | [[Category: Singh, S]] | + | [[Category: Jaiswal R]] |
| - | [[Category: Zaman, S]] | + | [[Category: Santosh V]] |
| - | [[Category: Dna binding protein-dna complex]] | + | [[Category: Singh S]] |
| - | [[Category: Protein-dna complex]] | + | [[Category: Zaman S]] |
| - | [[Category: Replication termination]]
| + | |
| - | [[Category: Transcription termination]]
| + | |
| Structural highlights
Function
REB1_SCHPO DNA-binding protein that recognizes sites within both the enhancer and the promoter of rRNA transcription, as well as upstream of many genes transcribed by RNA polymerase II. Has a role in the termination of RNA polymerase I catalyzed transcription.[1]
Publication Abstract from PubMed
Reb1 ofSchizosaccharomyces pomberepresents a family of multifunctional proteins that bind to specific terminator sites (Ter) and cause polar termination of transcription catalyzed by RNA polymerase I (pol I) and arrest of replication forks approaching the Ter sites from the opposite direction. However, it remains to be investigated whether the same mechanism causes arrest of both DNA transactions. Here, we present the structure of Reb1 as a complex with a Ter site at a resolution of 2.7 A. Structure-guided molecular genetic analyses revealed that it has distinct and well-defined DNA binding and transcription termination (TTD) domains. The region of the protein involved in replication termination is distinct from the TTD. Mechanistically, the data support the conclusion that transcription termination is not caused by just high affinity Reb1-Ter protein-DNA interactions. Rather, protein-protein interactions between the TTD with the Rpa12 subunit of RNA pol I seem to be an integral part of the mechanism. This conclusion is further supported by the observation that double mutations in TTD that abolished its interaction with Rpa12 also greatly reduced transcription termination thereby revealing a conduit for functional communications between RNA pol I and the terminator protein.
Functional architecture of the Reb1-Ter complex of Schizosaccharomyces pombe.,Jaiswal R, Choudhury M, Zaman S, Singh S, Santosh V, Bastia D, Escalante CR Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):E2267-76. doi:, 10.1073/pnas.1525465113. Epub 2016 Mar 28. PMID:27035982[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhao A, Guo A, Liu Z, Pape L. Molecular cloning and analysis of Schizosaccharomyces pombe Reb1p: sequence-specific recognition of two sites in the far upstream rDNA intergenic spacer. Nucleic Acids Res. 1997 Feb 15;25(4):904-10. PMID:9016645
- ↑ Jaiswal R, Choudhury M, Zaman S, Singh S, Santosh V, Bastia D, Escalante CR. Functional architecture of the Reb1-Ter complex of Schizosaccharomyces pombe. Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):E2267-76. doi:, 10.1073/pnas.1525465113. Epub 2016 Mar 28. PMID:27035982 doi:http://dx.doi.org/10.1073/pnas.1525465113
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