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6h5b

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Myxococcus xanthus MglA in complex with its GAP MglB and GTPgammaS

Structural highlights

6h5b is a 3 chain structure with sequence from Atcc 25232 and Myxxd. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , ,
Gene:	mglA, MXAN_1925 (MYXXD), mglB (ATCC 25232)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MGLA_MYXXD] Required for multicellular development and for both mechanisms of gliding: social (S) and adventurous (A) motility. Acts as an intracellular switch to coordinate A and S motilities. Controls the direction of gliding and gliding speed.^[1] ^[2]

Publication Abstract from PubMed

In Myxococcus xanthus, directed movement is controlled by pole-to-pole oscillations of the small GTPase MglA and its GAP MglB. Direction reversals require that MglA is inactivated by MglB, yet paradoxically MglA and MglB are located at opposite poles at reversal initiation. Here we report the complete MglA/MglB structural cycle combined to GAP kinetics and in vivo motility assays, which uncovers that MglA is a three-state GTPase and suggests a molecular mechanism for concerted MglA/MglB relocalizations. We show that MglA has an atypical GTP-bound state (MglA-GTP*) that is refractory to MglB and is re-sensitized by a feedback mechanism operated by MglA-GDP. By identifying and mutating the pole-binding region of MglB, we then provide evidence that the MglA-GTP* state exists in vivo. These data support a model in which MglA-GDP acts as a soluble messenger to convert polar MglA-GTP* into a diffusible MglA-GTP species that re-localizes to the opposite pole during reversals.

MglA functions as a three-state GTPase to control movement reversals of Myxococcus xanthus.,Galicia C, Lhospice S, Varela PF, Trapani S, Zhang W, Navaza J, Herrou J, Mignot T, Cherfils J Nat Commun. 2019 Nov 22;10(1):5300. doi: 10.1038/s41467-019-13274-3. PMID:31757955^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Spormann AM, Kaiser D. Gliding mutants of Myxococcus xanthus with high reversal frequencies and small displacements. J Bacteriol. 1999 Apr;181(8):2593-601. PMID:10198026
↑ Hartzell P, Kaiser D. Function of MglA, a 22-kilodalton protein essential for gliding in Myxococcus xanthus. J Bacteriol. 1991 Dec;173(23):7615-24. PMID:1938957
↑ Galicia C, Lhospice S, Varela PF, Trapani S, Zhang W, Navaza J, Herrou J, Mignot T, Cherfils J. MglA functions as a three-state GTPase to control movement reversals of Myxococcus xanthus. Nat Commun. 2019 Nov 22;10(1):5300. doi: 10.1038/s41467-019-13274-3. PMID:31757955 doi:http://dx.doi.org/10.1038/s41467-019-13274-3

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6h5b"

@@ Line 1: / Line 1: @@
-==Small GTPase in complex with its GAP==
+==Myxococcus xanthus MglA in complex with its GAP MglB and GTPgammaS==
 <StructureSection load='6h5b' size='340' side='right'caption='[[6h5b]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6h5b]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25232 Atcc 25232] and [http://en.wikipedia.org/wiki/Myxxd Myxxd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H5B FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6h5b]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25232 Atcc 25232] and [http://en.wikipedia.org/wiki/Myxxd Myxxd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5B OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6H5B FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GSP:5-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GSP:5-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mglA, MXAN_1925 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246197 MYXXD]), mglB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34 ATCC 25232])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h5b OCA], [http://pdbe.org/6h5b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h5b RCSB], [http://www.ebi.ac.uk/pdbsum/6h5b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h5b ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6h5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h5b OCA], [http://pdbe.org/6h5b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h5b RCSB], [http://www.ebi.ac.uk/pdbsum/6h5b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h5b ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 19: / Line 19: @@
 </div>
 <div class="pdbe-citations 6h5b" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Gliding protein|Gliding protein]]
 == References ==
 <references/>

6h5b

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Current revision

Myxococcus xanthus MglA in complex with its GAP MglB and GTPgammaS

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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