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| - | [[Image:1a1j.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1a1j.png|left|200px]] |
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| | {{STRUCTURE_1a1j| PDB=1a1j | SCENE= }} | | {{STRUCTURE_1a1j| PDB=1a1j | SCENE= }} |
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| - | '''RADR (ZIF268 VARIANT) ZINC FINGER-DNA COMPLEX (GCGT SITE)'''
| + | ===RADR (ZIF268 VARIANT) ZINC FINGER-DNA COMPLEX (GCGT SITE)=== |
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| - | ==Overview==
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| - | BACKGROUND: Zinc fingers of the Cys2-His2 class comprise one of the largest families of eukaryotic DNA-binding motifs and recognize a diverse set of DNA sequences. These proteins have a relatively simple modular structure and key base contacts are typically made by a few residues from each finger. These features make the zinc finger motif an attractive system for designing novel DNA-binding proteins and for exploring fundamental principles of protein-DNA recognition. RESULTS: Here we report the X-ray crystal structures of zinc finger-DNA complexes involving three variants of Zif268, with multiple changes in the recognition helix of finger one. We describe the structure of each of these three-finger peptides bound to its corresponding target site. To help elucidate the differential basis for site-specific recognition, the structures of four other complexes containing various combinations of these peptides with alternative binding sites have also been determined. CONCLUSIONS: The protein-DNA contacts observed in these complexes reveal the basis for the specificity demonstrated by these Zif268 variants. Many, but not all, of the contacts can be rationalized in terms of a recognition code, but the predictive value of such a code is limited. The structures illustrate how modest changes in the docking arrangement accommodate the new sidechain-base and sidechain-phosphate interactions. Such adaptations help explain the versatility of naturally occurring zinc finger proteins and their utility in design.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9562555}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9562555 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9562555}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Zinc finger]] | | [[Category: Zinc finger]] |
| | [[Category: Zinc finger-dna complex]] | | [[Category: Zinc finger-dna complex]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:40:10 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:47:27 2008'' |
Revision as of 12:47, 30 June 2008
Template:STRUCTURE 1a1j
RADR (ZIF268 VARIANT) ZINC FINGER-DNA COMPLEX (GCGT SITE)
Template:ABSTRACT PUBMED 9562555
About this Structure
1A1J is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
High-resolution structures of variant Zif268-DNA complexes: implications for understanding zinc finger-DNA recognition., Elrod-Erickson M, Benson TE, Pabo CO, Structure. 1998 Apr 15;6(4):451-64. PMID:9562555
Page seeded by OCA on Mon Jun 30 15:47:27 2008
