|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6pco' size='340' side='right'caption='[[6pco]], [[Resolution|resolution]] 2.75Å' scene=''> | | <StructureSection load='6pco' size='340' side='right'caption='[[6pco]], [[Resolution|resolution]] 2.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6pco]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"alcaligenes_bronchicanis"_(ferry_1911)_haupt_1935 "alcaligenes bronchicanis" (ferry 1911) haupt 1935]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PCO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6pco]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_bronchiseptica Bordetella bronchiseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PCO FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6pcp|6pcp]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BB1771 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=518 "Alcaligenes bronchicanis" (Ferry 1911) Haupt 1935])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pco OCA], [https://pdbe.org/6pco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pco RCSB], [https://www.ebi.ac.uk/pdbsum/6pco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pco ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pco OCA], [http://pdbe.org/6pco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pco RCSB], [http://www.ebi.ac.uk/pdbsum/6pco PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pco ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0H3LTT0_BORBR A0A0H3LTT0_BORBR] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 23: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Bordetella bronchiseptica]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Booth, W T]] | + | [[Category: Booth WT]] |
| - | [[Category: Davis, R R]] | + | [[Category: Davis RR]] |
| - | [[Category: Deora, R]] | + | [[Category: Deora R]] |
| - | [[Category: Hollis, T]] | + | [[Category: Hollis T]] |
| - | [[Category: Biofilm]]
| + | |
| - | [[Category: Bordetella]]
| + | |
| - | [[Category: Bpsr]]
| + | |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Inhibition]]
| + | |
| - | [[Category: Transcription regulation]]
| + | |
| Structural highlights
Function
A0A0H3LTT0_BORBR
Publication Abstract from PubMed
Bordetella bacteria are respiratory pathogens of humans, birds, and livestock. Bordetella pertussis the causative agent of whopping cough remains a significant health issue. The transcriptional regulator, BpsR, represses a number of Bordetella genes relating to virulence, cell adhesion, cell motility, and nicotinic acid metabolism. DNA binding of BpsR is allosterically regulated by interaction with 6-hydroxynicotinic acid (6HNA), the first product in the nicotinic acid degradation pathway. To understand the mechanism of this regulation, we have determined the crystal structures of BpsR and BpsR in complex with 6HNA. The structures reveal that BpsR binding of 6HNA induces a conformational change in the protein to prevent DNA binding. We have also identified homologs of BpsR in other Gram negative bacteria in which the amino acids involved in recognition of 6HNA are conserved, suggesting a similar mechanism for regulating nicotinic acid degradation.
Structural mechanism for regulation of DNA binding of BpsR, a Bordetella regulator of biofilm formation, by 6-hydroxynicotinic acid.,Booth WT, Davis RR, Deora R, Hollis T PLoS One. 2019 Nov 7;14(11):e0223387. doi: 10.1371/journal.pone.0223387., eCollection 2019. PMID:31697703[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Booth WT, Davis RR, Deora R, Hollis T. Structural mechanism for regulation of DNA binding of BpsR, a Bordetella regulator of biofilm formation, by 6-hydroxynicotinic acid. PLoS One. 2019 Nov 7;14(11):e0223387. doi: 10.1371/journal.pone.0223387., eCollection 2019. PMID:31697703 doi:http://dx.doi.org/10.1371/journal.pone.0223387
|
