6qvj

From Proteopedia

(Difference between revisions)

Revision as of 23:55, 6 March 2020

HsCKK (human CAMSAP1) decorated 14pf taxol-GDP microtubule

6qvj, resolution 3.80Å

Structural highlights

6qvj is a 5 chain structure with sequence from Human and Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	6qus, 6quy
Gene:	CAMSAP1 (HUMAN)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CAMP1_HUMAN] Probable microtubule-binding protein that plays a role in the regulation of cell morphology and cytoskeletal organization. Through interaction with spectrin may regulate neurite outgrowth.^[1] ^[2] ^[3] [TBB5_HUMAN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [TBA1B_HUMAN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Publication Abstract from PubMed

CAMSAP/Patronins regulate microtubule minus-end dynamics. Their end specificity is mediated by their CKK domains, which we proposed recognise specific tubulin conformations found at minus ends. To critically test this idea, we compared the human CAMSAP1 CKK domain (HsCKK) with a CKK domain from Naegleria gruberi (NgCKK), which lacks minus-end specificity. Here we report near-atomic cryo-electron microscopy structures of HsCKK- and NgCKK-microtubule complexes, which show that these CKK domains share the same protein fold, bind at the intradimer interprotofilament tubulin junction, but exhibit different footprints on microtubules. NMR experiments show that both HsCKK and NgCKK are remarkably rigid. However, whereas NgCKK binding does not alter the microtubule architecture, HsCKK remodels its microtubule interaction site and changes the underlying polymer structure because the tubulin lattice conformation is not optimal for its binding. Thus, in contrast to many MAPs, the HsCKK domain can differentiate subtly specific tubulin conformations to enable microtubule minus-end recognition.

Structural determinants of microtubule minus end preference in CAMSAP CKK domains.,Atherton J, Luo Y, Xiang S, Yang C, Rai A, Jiang K, Stangier M, Vemu A, Cook AD, Wang S, Roll-Mecak A, Steinmetz MO, Akhmanova A, Baldus M, Moores CA Nat Commun. 2019 Nov 20;10(1):5236. doi: 10.1038/s41467-019-13247-6. PMID:31748546^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Baines AJ, Bignone PA, King MD, Maggs AM, Bennett PM, Pinder JC, Phillips GW. The CKK domain (DUF1781) binds microtubules and defines the CAMSAP/ssp4 family of animal proteins. Mol Biol Evol. 2009 Sep;26(9):2005-14. doi: 10.1093/molbev/msp115. Epub 2009 Jun , 9. PMID:19508979 doi:http://dx.doi.org/10.1093/molbev/msp115
↑ Bai SW, Herrera-Abreu MT, Rohn JL, Racine V, Tajadura V, Suryavanshi N, Bechtel S, Wiemann S, Baum B, Ridley AJ. Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration. BMC Biol. 2011 Aug 11;9:54. doi: 10.1186/1741-7007-9-54. PMID:21834987 doi:10.1186/1741-7007-9-54
↑ King MD, Phillips GW, Bignone PA, Hayes NV, Pinder JC, Baines AJ. A conserved sequence in calmodulin regulated spectrin-associated protein 1 links its interaction with spectrin and calmodulin to neurite outgrowth. J Neurochem. 2014 Feb;128(3):391-402. doi: 10.1111/jnc.12462. Epub 2013 Oct 24. PMID:24117850 doi:http://dx.doi.org/10.1111/jnc.12462
↑ Atherton J, Luo Y, Xiang S, Yang C, Rai A, Jiang K, Stangier M, Vemu A, Cook AD, Wang S, Roll-Mecak A, Steinmetz MO, Akhmanova A, Baldus M, Moores CA. Structural determinants of microtubule minus end preference in CAMSAP CKK domains. Nat Commun. 2019 Nov 20;10(1):5236. doi: 10.1038/s41467-019-13247-6. PMID:31748546 doi:http://dx.doi.org/10.1038/s41467-019-13247-6

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6qvj"

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 ==HsCKK (human CAMSAP1) decorated 14pf taxol-GDP microtubule==
-<StructureSection load='6qvj' size='340' side='right'caption='[[6qvj]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
+<SX load='6qvj' size='340' side='right' viewer='molstar' caption='[[6qvj]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[6qvj]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QVJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QVJ FirstGlance]. <br>
@@ Line 23: / Line 23: @@
 <references/>
 __TOC__
-</StructureSection>
+</SX>
 [[Category: Homo sapiens]]
 [[Category: Human]]

6qvj

From Proteopedia

Revision as of 23:55, 6 March 2020

HsCKK (human CAMSAP1) decorated 14pf taxol-GDP microtubule

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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