1a1u

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[[Image:1a1u.gif|left|200px]]
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{{Seed}}
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[[Image:1a1u.png|left|200px]]
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{{STRUCTURE_1a1u| PDB=1a1u | SCENE= }}
{{STRUCTURE_1a1u| PDB=1a1u | SCENE= }}
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'''SOLUTION STRUCTURE DETERMINATION OF A P53 MUTANT DIMERIZATION DOMAIN, NMR, MINIMIZED AVERAGE STRUCTURE'''
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===SOLUTION STRUCTURE DETERMINATION OF A P53 MUTANT DIMERIZATION DOMAIN, NMR, MINIMIZED AVERAGE STRUCTURE===
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==Overview==
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The p53 tumor suppressor oligomerization domain, a dimer of two primary dimers, is an independently folding domain whose subunits consist of a beta-strand, a tight turn and an alpha-helix. To evaluate the effect of hydrophobic side-chains on three-dimensional structure, we substituted residues Phe341 and Leu344 in the alpha-helix with other hydrophobic amino acids. Substitutions that resulted in residue 341 having a smaller side-chain than residue 344 switched the stoichiometry of the domain from tetrameric to dimeric. The three-dimensional structure of one such dimer was determined by multidimensional NMR spectroscopy. When compared with the primary dimer of the wild-type p53 oligomerization domain, the mutant dimer showed a switch in alpha-helical packing from anti-parallel to parallel and rotation of the alpha-helices relative to the beta-strands. Hydrophobic side-chain size is therefore an important determinant of a protein fold.
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The line below this paragraph, {{ABSTRACT_PUBMED_9321402}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 9321402 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9321402}}
==About this Structure==
==About this Structure==
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1A1U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A1U OCA].
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1A1U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A1U OCA].
==Reference==
==Reference==
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[[Category: Oligomerization domain]]
[[Category: Oligomerization domain]]
[[Category: P53]]
[[Category: P53]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:40:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:48:34 2008''

Revision as of 12:48, 30 June 2008

Image:1a1u.png

Template:STRUCTURE 1a1u

SOLUTION STRUCTURE DETERMINATION OF A P53 MUTANT DIMERIZATION DOMAIN, NMR, MINIMIZED AVERAGE STRUCTURE

Template:ABSTRACT PUBMED 9321402

About this Structure

1A1U is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Hydrophobic side-chain size is a determinant of the three-dimensional structure of the p53 oligomerization domain., McCoy M, Stavridi ES, Waterman JL, Wieczorek AM, Opella SJ, Halazonetis TD, EMBO J. 1997 Oct 15;16(20):6230-6. PMID:9321402

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