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1a2x

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{{STRUCTURE_1a2x| PDB=1a2x | SCENE= }}
{{STRUCTURE_1a2x| PDB=1a2x | SCENE= }}
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'''COMPLEX OF TROPONIN C WITH A 47 RESIDUE (1-47) FRAGMENT OF TROPONIN I'''
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===COMPLEX OF TROPONIN C WITH A 47 RESIDUE (1-47) FRAGMENT OF TROPONIN I===
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==Overview==
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Troponin (Tn), the complex of three subunits (TnC, TnI, and TnT), plays a key role in Ca2+-dependent regulation of muscle contraction. To elucidate the interactions between the Tn subunits and the conformation of TnC in the Tn complex, we have determined the crystal structure of TnC (two Ca2+ bound state) in complex with the N-terminal fragment of TnI (TnI1-47). The structure was solved by the single isomorphous replacement method in combination with multiple wavelength anomalous dispersion data. The refinement converged to a crystallographic R factor of 22.2% (Rfree = 32.6%). The central, connecting alpha-helix observed in the structure of uncomplexed TnC (TnCfree) is unwound at the center (residues Ala-87, Lys-88, Gly-89, Lys-90, and Ser-91) and bent by 90 degrees. As a result, TnC in the complex has a compact globular shape with direct interactions between the N- and C-terminal lobes, in contrast to the elongated dumb-bell shaped molecule of uncomplexed TnC. The 31-residue long TnI1-47 alpha-helix stretches on the surface of TnC and stabilizes its compact conformation by multiple contacts with both TnC lobes. The amphiphilic C-end of the TnI1-47 alpha-helix is bound in the hydrophobic pocket of the TnC C-lobe through 38 van der Waals interactions. The results indicate the major difference between Ca2+ receptors integrated with the other proteins (TnC in Tn) and isolated in the cytosol (calmodulin). The TnC/TnI1-47 structure implies a mechanism of how Tn regulates the muscle contraction and suggests a unique alpha-helical regulatory TnI segment, which binds to the N-lobe of TnC in its Ca2+ bound conformation.
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{{ABSTRACT_PUBMED_9560191}}
==About this Structure==
==About this Structure==
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[[Category: Muscle contraction regulation]]
[[Category: Muscle contraction regulation]]
[[Category: Troponin]]
[[Category: Troponin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:53:02 2008''

Revision as of 12:53, 30 June 2008

Image:1a2x.png

Template:STRUCTURE 1a2x

COMPLEX OF TROPONIN C WITH A 47 RESIDUE (1-47) FRAGMENT OF TROPONIN I

Template:ABSTRACT PUBMED 9560191

About this Structure

1A2X is a Protein complex structure of sequences from Oryctolagus cuniculus. Full crystallographic information is available from OCA.

Reference

Crystal structure of troponin C in complex with troponin I fragment at 2.3-A resolution., Vassylyev DG, Takeda S, Wakatsuki S, Maeda K, Maeda Y, Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):4847-52. PMID:9560191

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