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4x1o

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Revision as of 21:07, 12 April 2023

Crystal structure of the 16S rRNA (adenine(1408)-N(1))-methyltransferase from Catenulisporales acidiphilia

Structural highlights

4x1o is a 1 chain structure with sequence from Catenulispora acidiphila DSM 44928. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

C7Q5P8_CATAD

Publication Abstract from PubMed

Methylation of the bacterial small ribosomal subunit (16S) rRNA on the N1 position of A1408 confers exceptionally high-level resistance to a broad spectrum of aminoglycoside antibiotics. Here, we present a detailed structural and functional analysis of the Catenulisporales acidiphilia 16S rRNA (m(1)A1408) methyltransferase ('CacKam'). The apo CacKam structure closely resembles other m(1)A1408 methyltransferases within its conserved SAM-binding fold but the region linking core beta strands 6 and 7 (the 'beta6/7 linker') has a unique, extended structure that partially occludes the putative 16S rRNA binding surface, and sequesters the conserved and functionally critical W203 outside of the CacKam active site. Substitution of conserved residues in the SAM binding pocket reveals a functional dichotomy in the 16S rRNA (m(1)A1408) methyltransferase family, with two apparently distinct molecular mechanisms coupling cosubstrate/ substrate binding to catalytic activity. Our results additionally suggest that CacKam exploits the W203-mediated remodeling of the beta6/7 linker as a novel mechanism to control 30S substrate recognition and enzymatic turnover.

Functional dichotomy in the 16S rRNA (m1A1408) methyltransferase family and control of catalytic activity via a novel tryptophan mediated loop reorganization.,Witek MA, Conn GL Nucleic Acids Res. 2016 Jan 8;44(1):342-53. doi: 10.1093/nar/gkv1306. Epub 2015, Nov 24. PMID:26609134^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Witek MA, Conn GL. Functional dichotomy in the 16S rRNA (m1A1408) methyltransferase family and control of catalytic activity via a novel tryptophan mediated loop reorganization. Nucleic Acids Res. 2016 Jan 8;44(1):342-53. doi: 10.1093/nar/gkv1306. Epub 2015, Nov 24. PMID:26609134 doi:http://dx.doi.org/10.1093/nar/gkv1306

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4x1o"

Categories: Catenulispora acidiphila DSM 44928 | Large Structures | Conn GL | Witek MA

@@ Line 3: / Line 3: @@
 <StructureSection load='4x1o' size='340' side='right'caption='[[4x1o]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4x1o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Catad Catad]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X1O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X1O FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4x1o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Catenulispora_acidiphila_DSM_44928 Catenulispora acidiphila DSM 44928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X1O FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Caci_9046 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=479433 CATAD])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x1o OCA], [https://pdbe.org/4x1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x1o RCSB], [https://www.ebi.ac.uk/pdbsum/4x1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x1o ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x1o OCA], [http://pdbe.org/4x1o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x1o RCSB], [http://www.ebi.ac.uk/pdbsum/4x1o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x1o ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/C7Q5P8_CATAD C7Q5P8_CATAD]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Catad]]
+[[Category: Catenulispora acidiphila DSM 44928]]
 [[Category: Large Structures]]
-[[Category: Conn, G L]]
+[[Category: Conn GL]]
-[[Category: Witek, M A]]
+[[Category: Witek MA]]
-[[Category: Aminoglycoside resistance]]
-[[Category: Methyltransferase]]
-[[Category: Ribosome]]
-[[Category: Transferase]]

4x1o

From Proteopedia

Revision as of 21:07, 12 April 2023

Crystal structure of the 16S rRNA (adenine(1408)-N(1))-methyltransferase from Catenulisporales acidiphilia

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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